Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells.
Details
Serval ID
serval:BIB_DA6B8FFFB72D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Effect of laparoscopy versus laparotomy on circulating tumor cells using isolation by size of epithelial tumor cells.
Journal
International journal of gynecological cancer
ISSN
1048-891X (Print)
ISSN-L
1048-891X
Publication state
Published
Issued date
2004
Peer-reviewed
Oui
Volume
14
Number
2
Pages
229-233
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
To assess the effect of laparoscopy on circulating tumor cell (CTC) detection in case of carcinosis.
We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO(2) laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.
Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one post-experiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).
This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.
We compared laparoscopy versus laparotomy on tumor cell blood release in an animal model of ovarian carcinosis obtained by intraperitoneal inoculation of IGR-OV1 cells in nude rats. Animals were randomly assigned to one of the following groups: CO(2) laparoscopy (L), gasless laparoscopy (GL), midline laparotomy (ML), or general anesthesia as control (C). A 0.5 ml blood sample was taken in each case before and after experiment and tested with a novel assay, ISET (isolation by size of epithelial tumor cells), which isolates CTC by filtration on account of their size. Statistics were performed with the Fisher's and the Chi-square tests.
Ten rats were included in each group. We did not find any significant difference in CTC prevalence before and after surgery (2/14 versus 3/19, respectively, P = 1). Similarly, the three surgical accesses were equivalent with one post-experiment detection per group: 1/5 for L, 1/7 for ML, 1/7 for GL, and 1/6 for C (P = 0.9).
This trial did not show any deleterious effect of laparoscopy on CTC when compared to laparotomy.
Keywords
Animals, Disease Models, Animal, Female, Laparoscopy/adverse effects, Laparotomy/adverse effects, Neoplasm Transplantation, Neoplastic Cells, Circulating/pathology, Ovarian Neoplasms/pathology, Ovarian Neoplasms/surgery, Random Allocation, Rats, Rats, Nude
Pubmed
Web of science
Create date
31/10/2019 18:22
Last modification date
05/02/2021 7:26
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