Collagen response and glycoprotein VI function decline progressively as canine platelets age in vivo.

Details

Serval ID
serval:BIB_DA2D810EF7C8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Collagen response and glycoprotein VI function decline progressively as canine platelets age in vivo.
Journal
Thrombosis and Haemostasis
Author(s)
Alberio L., Friese P., Clemetson K.J., Dale G.L.
ISSN
0340-6245 (Print)
ISSN-L
0340-6245
Publication state
Published
Issued date
2002
Peer-reviewed
Oui
Volume
88
Number
3
Pages
510-516
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S. Publication Status: ppublish
Abstract
Clinical and experimental observations suggest that platelet function deteriorates quickly with cell age. However, efforts to define age-dependent alterations have detected only modest biochemical changes occurring late in the cell life span. In this report, we demonstrate two significant alterations of the collagen response occurring during in vivo aging of canine platelets: a progressive increase in the EC50 for collagen types I, III and V and the emergence of a population of aged platelets which are refractory to collagen. Experiments with convulxin, a specific agonist for the collagen receptor glycoprotein VI (GPVI), also demonstrate an age-dependent decline in activation and the appearance of a non-reactive, aged population as observed with native collagens. Our studies indicate that canine platelets have two distinct binding levels for FITC-labeled convulxin and that the higher binding level disappears upon cell aging. During these studies one dog (#428) was identified whose platelets not only failed to demonstrate an age-dependent decrease in convulxin reactivity but also maintained a high convulxin-binding ability throughout their otherwise normal life span. Transfusion of biotinylated platelets from control dogs into dog #428 showed that the expected changes in collagen response and GPVI function did not occur in the transfused platelets. These observations demonstrate that the canine platelet response towards collagen is strongly dependent upon cell-age and suggest that this functional decline is at least partly due to an extrinsic-mediated alteration, possibly proteolytic, of GPVI.
Keywords
Animals, Blood Platelets/physiology, Cell Aging, Collagen/metabolism, Collagen Type I/metabolism, Collagen Type III/metabolism, Collagen Type V/metabolism, Crotalid Venoms/pharmacology, Dogs, Lectins, C-Type, P-Selectin/analysis, Platelet Activation, Platelet Adhesiveness, Platelet Membrane Glycoproteins/agonists, Platelet Membrane Glycoproteins/physiology
Pubmed
Web of science
Create date
10/02/2015 11:03
Last modification date
20/08/2019 15:59
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