Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.
Details
Serval ID
serval:BIB_D9CBF28FFC6D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Viral Q beta RNA as a high expression vector for mRNA translation in a cell-free system.
Journal
FEBS Letters
ISSN
0014-5793 (Print)
ISSN-L
0014-5793
Publication state
Published
Issued date
1995
Volume
359
Number
1
Pages
89-92
Language
english
Abstract
Dihydrofolate reductase (DHFR) mRNA was inserted into Q beta phage RNA instead of its coat protein cistron. Translation of this recombinant mRNA in the Escherichia coli cell-free system resulted in the synthesis of DHFR, which was two orders of magnitude higher than that in the case of translation of the control DHFR mRNA. Additionally, it resulted in a significantly enhanced synthesis of Q beta replicase as compared with its synthesis when the original Q beta RNA was used.
Keywords
Allolevivirus/genetics, Capsid/genetics, Escherichia coli/genetics, Gene Transfer Techniques, Genes, Genetic Vectors, Protein Biosynthesis, RNA, Messenger/genetics, RNA, Viral/genetics, Recombinant Proteins/biosynthesis, Tetrahydrofolate Dehydrogenase/biosynthesis, Tetrahydrofolate Dehydrogenase/genetics
Pubmed
Web of science
Open Access
Yes
Create date
09/07/2012 10:16
Last modification date
20/08/2019 16:59
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