High-Resolution Tissue Mass Spectrometry Imaging Reveals a Refined Functional Anatomy of the Human Adult Adrenal Gland.

Details

Serval ID
serval:BIB_D9700A839DF4
Type
Article: article from journal or magazin.
Collection
Publications
Title
High-Resolution Tissue Mass Spectrometry Imaging Reveals a Refined Functional Anatomy of the Human Adult Adrenal Gland.
Journal
Endocrinology
Author(s)
Sun N., Wu Y., Nanba K., Sbiera S., Kircher S., Kunzke T., Aichler M., Berezowska S., Reibetanz J., Rainey W.E., Fassnacht M., Walch A., Kroiss M.
ISSN
1945-7170 (Electronic)
ISSN-L
0013-7227
Publication state
Published
Issued date
01/03/2018
Peer-reviewed
Oui
Volume
159
Number
3
Pages
1511-1524
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In the adrenal gland, neuroendocrine cells that synthesize catecholamines and epithelial cells that produce steroid hormones are united beneath a common organ capsule to function as a single stress-responsive organ. The functional anatomy of the steroid hormone-producing adrenal cortex and the catecholamine-producing medulla is ill defined at the level of small molecules. Here, we report a comprehensive high-resolution mass spectrometry imaging (MSI) map of the normal human adrenal gland. A large variety of biomolecules was accessible by matrix-assisted laser desorption/ionization-Fourier transform-ion cyclotron resonance MSI, including nucleoside phosphates indicative of oxidative phosphorylation, sterol and steroid metabolites, intermediates of glycolysis and the tricarboxylic acid cycle, lipids, and fatty acids. Statistical clustering analyses yielded a molecularly defined adrenal anatomy of 10 distinct molecular zones including a highly structured corticomedullary interface. By incorporating pathway information, activities of carbohydrate, amino acid, and lipid metabolism as well as endocrine bioactivity were revealed to be highly spatially organized, which could be visualized as different molecularly defined zones. Together, these findings provide a molecular definition of human adult adrenal gland structure beyond classical histological anatomy.
Keywords
Adrenal Glands/anatomy & histology, Adrenal Glands/chemistry, Adrenal Glands/metabolism, Adult, Amino Acids/metabolism, Carbohydrate Metabolism, Female, Humans, Immunohistochemistry, Lipid Metabolism, Male, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Young Adult
Pubmed
Web of science
Open Access
Yes
Create date
29/06/2020 9:34
Last modification date
30/06/2020 5:26
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