Mice that lack the interferon-gamma receptor have profoundly altered responses to infection with Bacillus Calmette-Guérin and subsequent challenge with lipopolysaccharide.

Details

Serval ID
serval:BIB_D9642E7B8E0C
Type
Article: article from journal or magazin.
Collection
Publications
Title
Mice that lack the interferon-gamma receptor have profoundly altered responses to infection with Bacillus Calmette-Guérin and subsequent challenge with lipopolysaccharide.
Journal
Journal of Experimental Medicine
Author(s)
Kamijo R., Le J., Shapiro D., Havell E.A., Huang S., Aguet M., Bosland M., Vilcek J.
ISSN
0022-1007 (Print)
ISSN-L
0022-1007
Publication state
Published
Issued date
1993
Volume
178
Number
4
Pages
1435-1440
Language
english
Abstract
Mice with a targeted disruption of the interferon gamma receptor gene (IFN-gamma R0/0 mice) and control wild-type mice were inoculated with the Bacillus Calmette-Guérin (BCG) strain of Mycobacterium bovis. BCG infection was not lethal for wild-type mice whereas all IFN-gamma R0/0 mice died approximately 7-9 wk after inoculation. Histological examination at 2 and 6 wk after BCG inoculation showed that livers of IFN-gamma R0/0 mice had higher numbers of acid-fast bacteria than wild-type mice, especially at 6 wk. In parallel, the livers of IFN-gamma R0/0 mice showed a reduction in the formation of characteristic granulomas at 2 wk after inoculation. Injection of lipopolysaccharide (LPS) 2 wk after BCG inoculation was significantly less lethal for IFN-gamma R0/0 mice than for wild-type mice. Reduced lethality of LPS correlated with a drastically reduced production of tumor necrosis factor alpha (TNF-alpha) in the IFN-gamma R0/0 mice. Interleukin 1 alpha (IL-1 alpha) and IL-6 levels in the serum were also significantly reduced in the IFN-gamma R0/0 mice after BCG infection and LPS challenge. The greatly reduced capacity of BCG-infected IFN-gamma R0/0 mice to produce TNF-alpha may be an important factor in their inability to resist BCG infection. These results show that the presence of a functional IFN-gamma receptor is essential for the recovery of mice from BCG infection, and that IFN-gamma is a key element in the complex process whereby BCG infection leads to the sensitization to endotoxin.
Keywords
Animals, Cytokines/biosynthesis, Endotoxins/toxicity, Granuloma/microbiology, Immunity, Innate, Lipopolysaccharides/pharmacology, Liver Diseases/microbiology, Liver Diseases/pathology, Mice, Mice, Inbred C57BL, Mycobacterium bovis/immunology, Receptors, Interferon/genetics, Receptors, Interferon/physiology, Tuberculosis/immunology, Tuberculosis/mortality, Tumor Necrosis Factor-alpha/biosynthesis
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:37
Last modification date
20/08/2019 15:58
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