Field-emission scanning electron microscopy of the internal cellular organization of fungi.

Details

Serval ID
serval:BIB_D899FE3FEB4D
Type
Article: article from journal or magazin.
Collection
Publications
Title
Field-emission scanning electron microscopy of the internal cellular organization of fungi.
Journal
Scanning
Author(s)
Müller W.H., van Aelst A.C., Humbel B.M., van der Krift T.P., Boekhout T.
ISSN
0161-0457 (Print)
ISSN-L
0161-0457
Publication state
Published
Issued date
2000
Volume
22
Number
5
Pages
295-303
Language
english
Abstract
Internal viewing of the cellular organization of hyphae by scanning electron microscopy is an alternative to observing sectioned fungal material with a transmission electron microscope. To study cytoplasmic organelles in the hyphal cells of fungi by SEM, colonies were chemically fixed with glutaraldehyde and osmium tetroxide and then immersed in dimethyl sulfoxide. Following this procedure, the colonies were frozen and fractured on a liquid nitrogen-precooled metal block. Next, the fractured samples were macerated in diluted osmium tetroxide to remove the cytoplasmic matrix and subsequently dehydrated by freeze substitution in methanol. After critical point drying, mounting, and sputter coating, fractured cells of several basidiomycetes were imaged with field-emission SEM. This procedure produced clear images of elongated and spherical mitochondria, the nucleus, intravacuolar structures, tubular- and plate-like endoplasmic reticulum, and different types of septal pore caps. This method is a powerful approach for studying the intracellular ultrastructure of fungi by SEM.
Keywords
Fungi/ultrastructure, Microscopy, Electron, Scanning
Pubmed
Web of science
Open Access
Yes
Create date
18/10/2012 14:10
Last modification date
20/08/2019 15:58
Usage data