Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres.

Details

Serval ID
serval:BIB_D8663AD910A9
Type
Article: article from journal or magazin.
Collection
Publications
Title
Release of endogenous amino acids, including homocysteic acid and cysteine sulphinic acid, from rat hippocampal slices evoked by electrical stimulation of Schaffer collateral-commissural fibres.
Journal
Neuroscience
Author(s)
Klancnik J.M., Cuénod M., Gähwiler B.H., Jiang Z.P., Do K.Q.
ISSN
0306-4522 (Print)
ISSN-L
0306-4522
Publication state
Published
Issued date
1992
Peer-reviewed
Oui
Volume
49
Number
3
Pages
557-570
Language
english
Notes
Publication types: In Vitro ; Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
This study examined the release of endogenous amino acids from acute hippocampal slices, upon stimulation of the Schaffer collateral-commissural fibres. One-minute samples of superfusate were collected via a cannula placed over the CA1 stratum radiatum, and were analysed by reversed-phase high performance liquid chromatography. Evoked potentials were recorded to ascertain stimulation efficacy. Four minutes of continuous 50 Hz stimulation produced a tetrodotoxin-sensitive release of aspartate and glycine in the second minute of stimulation, as well as a tetrodotoxin-sensitive release of cysteine sulphinic acid, during stimulation and of homocysteic acid, following stimulation. Such 50 Hz stimulation also produced a tetrodotoxin-insensitive decrease in methionine levels, but no significant changes in any of the other 15 amino acids measured. Four minutes of continuous 1 Hz stimulation produced no changes in the levels of any of the amino acids measured, but four 600-ms trains of 100 Hz stimulation, which, unlike the 1 Hz stimulation, produced long-term potentiation, resulted in significant increases in levels of cysteine sulphinic acid and homocysteic acid, but not of any of the other amino acids measured. These results suggest that aspartate, glycine, homocysteic acid, and cysteine sulphinic acid play a role in synaptic transmission in the Schaffer collateral-commissural fibres, and that cysteine sulphinic acid and homocysteic acid may be released specifically by high-frequency stimulation.
Keywords
Amino Acids/metabolism, Animals, Aspartic Acid/metabolism, Cysteine/analogs & derivatives, Cysteine/metabolism, Electric Stimulation, Evoked Potentials, Glutamates/metabolism, Glutamic Acid, Glycine/metabolism, Hippocampus/physiology, Homocysteine/analogs & derivatives, Homocysteine/metabolism, Kinetics, Male, Methionine/metabolism, Nerve Fibers/physiology, Neurotransmitter Agents, Pyramidal Tracts/physiology, Rats, Rats, Inbred Strains, Time Factors, gamma-Aminobutyric Acid/metabolism
Pubmed
Web of science
Create date
06/03/2014 16:45
Last modification date
20/08/2019 15:57
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