[Retracted Publication] Non-random, individual-specific methylation profiles are present at the sixth CTCF binding site in the human H19/IGF2 imprinting control region.

Details

This publication has been retracted.
Serval ID
serval:BIB_D83A92F7CB3D
Type
Article: article from journal or magazin.
Collection
Publications
Title
[Retracted Publication] Non-random, individual-specific methylation profiles are present at the sixth CTCF binding site in the human H19/IGF2 imprinting control region.
Journal
Nucleic Acids Research
Author(s)
Tost J., Jammes H., Dupont J.M., Buffat C., Robert B., Mignot T.M., Mondon F., Carbonne B., Siméoni U., Grangé G., Kerjean A., Ferré F., Gut I.G., Vaiman D.
ISSN
1362-4962 (Electronic)
ISSN-L
0305-1048
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
34
Number
19
Pages
5438-5448
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Retracted Publication Publication Status: ppublish
Retraction in
Non-random, individual-specific methylation profiles are present at the sixth CTCF binding site in the human H19/IGF2 imprinting control region. [Nucleic Acids Res. 2007]
PMID: 17012269
Abstract
Expression of imprinted genes is classically associated with differential methylation of specific CpG-rich DNA regions (DMRs). The H19/IGF2 locus is considered a paradigm for epigenetic regulation. In mice, as in humans, the essential H19 DMR--target of the CTCF insulator--is located between the two genes. Here, we performed a pyrosequencing-based quantitative analysis of its CpG methylation in normal human tissues. The quantitative analysis of the methylation level in the H19 DMR revealed three unexpected discrete, individual-specific methylation states. This epigenetic polymorphism was confined to the sixth CTCF binding site while a unique median-methylated profile was found at the third CTCF binding site as well as in the H19 promoter. Monoallelic expression of H19 and IGF2 was maintained independently of the methylation status at the sixth CTCF binding site and the IGF2 DMR2 displayed a median-methylated profile in all individuals and tissues analyzed. Interestingly, the methylation profile was genetically transmitted. Transgenerational inheritance of the H19 methylation profile was compatible with a simple model involving one gene with three alleles. The existence of three individual-specific epigenotypes in the H19 DMR in a non-pathological situation means it is important to reconsider the diagnostic value and functional importance of the sixth CTCF binding site.
Keywords
Binding Sites, CpG Islands, DNA Methylation, DNA-Binding Proteins/metabolism, Female, Gene Expression, Genomic Imprinting, Genotype, Humans, Infant, Newborn, Inheritance Patterns, Insulin-Like Growth Factor II, Male, Models, Genetic, Pedigree, Placenta/metabolism, Polymerase Chain Reaction, Proteins/genetics, Proteins/metabolism, RNA, Long Noncoding, RNA, Untranslated/genetics, RNA, Untranslated/metabolism, Regulatory Sequences, Nucleic Acid, Repressor Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
22/02/2015 11:58
Last modification date
24/08/2020 9:48
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