Characterization of the cell type-specific determinant in the genome of minute virus of mice

Details

Serval ID
serval:BIB_D83256B2B908
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Characterization of the cell type-specific determinant in the genome of minute virus of mice
Journal
Journal of Virology
Author(s)
Antonietti  J. P., Sahli  R., Beard  P., Hirt  B.
ISSN
0022-538X (Print)
Publication state
Published
Issued date
02/1988
Volume
62
Number
2
Pages
552-7
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb
Abstract
Two strains of minute virus of mice (MVM) show different host cell specificities. The prototype strain MVM(p) grows in fibroblasts, whereas the immunosuppressive variant MVM(i) grows in T lymphocytes. In this study, we have mapped on the viral genome a cell type-specific determinant: it is located between 69 and 85 map units in a region coding for the viral capsid proteins. The DNA of MVM(p) does not replicate in lymphocytes. MVM(i) cannot help MVM(p) grow in lymphocytes; thus the determinant acts in a cis fashion. We did not detect viral mRNA during a restrictive infection of lymphocytes with MVM(p). However, when the same cells were transfected with cloned DNA, both MVM(p) and MVM(i) DNAs were transcribed with the same efficiency from both promoters and the RNA was processed normally. Therefore, the specificity determinant is not a cell type-specific enhancer.
Keywords
Cell Line Cloning, Molecular DNA Replication DNA, Viral/biosynthesis/genetics Fibroblasts *Genes, Viral Minute virus of mice/*genetics/growth & development Nucleic Acid Hybridization Parvoviridae/*genetics RNA, Messenger/genetics RNA, Viral/genetics T-Lymphocytes/*microbiology Transcription, Genetic Transfection Virus Replication
Pubmed
Web of science
Create date
24/01/2008 20:51
Last modification date
20/08/2019 15:57
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