Full activation of the T cell receptor requires both clustering and conformational changes at CD3.

Details

Serval ID
serval:BIB_D82E97FCBD75
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Full activation of the T cell receptor requires both clustering and conformational changes at CD3.
Journal
Immunity
Author(s)
Minguet S., Swamy M., Alarcón B., Luescher I.F., Schamel W.W.
ISSN
1074-7613
Publication state
Published
Issued date
2007
Peer-reviewed
Oui
Volume
26
Number
1
Pages
43-54
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
T cell receptor (TCR-CD3) triggering involves both receptor clustering and conformational changes at the cytoplasmic tails of the CD3 subunits. The mechanism by which TCRalphabeta ligand binding confers conformational changes to CD3 is unknown. By using well-defined ligands, we showed that induction of the conformational change requires both multivalent engagement and the mobility restriction of the TCR-CD3 imposed by the plasma membrane. The conformational change is elicited by cooperative rearrangements of two TCR-CD3 complexes and does not require accompanying changes in the structure of the TCRalphabeta ectodomains. This conformational change at CD3 reverts upon ligand dissociation and is required for T cell activation. Thus, our permissive geometry model provides a molecular mechanism that rationalizes how the information of ligand binding to TCRalphabeta is transmitted to the CD3 subunits and to the intracellular signaling machinery.
Keywords
Animals, Antigens, CD3/chemistry, Antigens, CD3/immunology, Humans, Immunoblotting, Immunoprecipitation, Lymphocyte Activation/immunology, Mice, Receptors, Antigen, T-Cell/chemistry, Receptors, Antigen, T-Cell/immunology, T-Lymphocytes/immunology, Transfection
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 11:19
Last modification date
20/08/2019 15:57
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