Abdominal irradiation increases inflammatory cytokine expression and activates NF-kappaB in rat ileal muscularis layer.

Details

Serval ID
serval:BIB_D8222AB407B2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Abdominal irradiation increases inflammatory cytokine expression and activates NF-kappaB in rat ileal muscularis layer.
Journal
American journal of physiology. Gastrointestinal and liver physiology
Author(s)
Linard C., Ropenga A., Vozenin-Brotons M.C., Chapel A., Mathe D.
ISSN
0193-1857 (Print)
ISSN-L
0193-1857
Publication state
Published
Issued date
09/2003
Peer-reviewed
Oui
Volume
285
Number
3
Pages
G556-65
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
The small bowel is an important dose-limiting organ in abdominal radiotherapy because irradiation can cause acute enteritis that, in turn, leads to progressively reduced motility and finally, in a later phase, to fibrosis. Because these clinical symptoms may be caused by the early stage of an inflammatory process, we characterized the radiation-induced intestinal inflammation in rats. Abdominal gamma-irradiation (10-Gy) induced a cascade of inflammatory events characterized by an early (6 h after exposure) increase in IL-1beta, TNF-alpha, and IL-6 mRNA levels in the rat ileal muscularis layer. IL-8 [a cytokine-induced neutrophil chemoattractant (CINC)] mRNA appeared later (at 3 days). The expression of TGF-beta (a profibrotic cytokine) was higher in irradiated than control tissue at day 1, whereas IL-10 (an anti-inflammatory cytokine) expression vanished completely. Despite strong IL-1ra expression, the IL-1ra/IL-1beta ratio, which is an indicator of inflammatory balance, was -41% at day 1 in irradiated compared with control tissue. The nuclear transcription factors NF-kappaB and activator protein-1 (AP-1) govern transcription of these genes, directly or indirectly. Although expression of the subunits of NF-kappaB (p65, p50) and AP-1 (c-fos, c-jun) did not increase, irradiation caused a rapid and persistent translocation of p65 and p50. An imbalance between proinflammatory and anti-inflammatory mediators may contribute to perpetuating intestinal inflammation, thus making it chronic.

Keywords
Abdomen/radiation effects, Animals, Cytokines/genetics, Cytokines/metabolism, Cytokines/radiation effects, Ileum/metabolism, Inflammation Mediators/metabolism, Inflammation Mediators/radiation effects, Male, Muscle, Smooth/metabolism, NF-kappa B/metabolism, NF-kappa B/physiology, NF-kappa B/radiation effects, Protein Isoforms/metabolism, RNA, Messenger/metabolism, Rats, Rats, Wistar, Transcription Factor AP-1/metabolism
Pubmed
Web of science
Create date
27/04/2018 15:39
Last modification date
20/08/2019 15:57
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