Beta-lactam resistance mechanisms of methicillin-resistant Staphylococcus aureus.

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It was possible to publish this article open access thanks to a Swiss National Licence with the publisher.
Serval ID
serval:BIB_D80288903E3E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Beta-lactam resistance mechanisms of methicillin-resistant Staphylococcus aureus.
Journal
Journal of Infectious Diseases
Author(s)
Franciolli M., Bille J., Glauser M.P., Moreillon P.
ISSN
0022-1899 (Print)
ISSN-L
0022-1899
Publication state
Published
Issued date
1991
Volume
163
Number
3
Pages
514-523
Language
english
Abstract
In vitro and in vivo activity of amoxicillin and penicillin G alone or combined with a penicillinase inhibitor (clavulanate) were tested against five isogenic pairs of methicillin-resistant Staphylococcus aureus (MRSA) producing or not producing penicillinase. Loss of the penicillinase plasmid caused an eight times or greater reduction in the MICs of amoxicillin and penicillin G (from greater than or equal to 64 to 8 micrograms/ml), but not of the penicillinase-resistant drugs methicillin and cloxacillin (greater than or equal to 64 micrograms/ml). This difference in antibacterial effectiveness correlated with a more than 10 times greater penicillin-binding protein 2a affinity of amoxicillin and penicillin G than of methicillin and a greater than or equal to 90% successful amoxicillin treatment of experimental endocarditis due to penicillinase-negative MRSA compared with cloxacillin, which was totally ineffective (P less than .001). Amoxicillin was also effective against penicillinase-producing parent MRSA, provided it was combined with clavulanate. Penicillinase-sensitive beta-lactam antibiotics plus penicillinase inhibitors might offer a rational alternative treatment for MRSA infections.
Keywords
Animals, Anti-Bacterial Agents/metabolism, Anti-Bacterial Agents/pharmacology, Bacterial Proteins/metabolism, Binding, Competitive, Carrier Proteins/metabolism, Drug Resistance, Microbial, Endocarditis, Bacterial/blood, Endocarditis, Bacterial/drug therapy, Female, Hexosyltransferases, Methicillin Resistance, Microbial Sensitivity Tests, Muramoylpentapeptide Carboxypeptidase/metabolism, Penicillin-Binding Proteins, Penicillinase, Peptidyl Transferases, Protein Binding, Rats, Rats, Inbred Strains, Staphylococcal Infections/blood, Staphylococcal Infections/drug therapy, Staphylococcus aureus/drug effects, Staphylococcus aureus/enzymology, beta-Lactams
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:57
Last modification date
01/10/2019 6:19
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