Sondes nucléiques et PCR: applications au diagnostic des infections bactériennes [Nucleic probes and PCR: application in the diagnosis of bacterial infections].

Details

Serval ID
serval:BIB_D7E0F5DB5B6D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Sondes nucléiques et PCR: applications au diagnostic des infections bactériennes [Nucleic probes and PCR: application in the diagnosis of bacterial infections].
Journal
Schweizerische Rundschau für Medizin Praxis = Revue Suisse de Médecine Praxis
Author(s)
Bille J., Ogay K.J., Ninet B.
ISSN
1013-2058 (Print)
ISSN-L
1013-2058
Publication state
Published
Issued date
1994
Volume
83
Number
20
Pages
609-614
Language
french
Notes
Publication types: English Abstract ; Journal Article
Publication Status: ppublish
Abstract
Probes and amplification systems represent new technologic developments in the domain of clinical microbiology. In spite of their high specificity, simple applicability and high velocity the DNA probes show only little sensitivity especially for the direct detection of microorganisms in clinical samples. This sensitivity lead to the development of DNA-amplification technics. PCR is the most famous of them and the most often used one. Next to the direct detection of microorganisms in clinical samples without culture these technics also allow the direct isolation of bacterial toxins, of genes for resistance to antibiotics as well as a systematic search for pathogens in diseases with unknown cause. Different problems of these methods in daily clinical practice are illustrated by four applications 1. direct detection of mycobacterium tuberculosis complex (M. tbc) in clinical samples, 2. rapid identification of mycobacterial species, 3. their typing for epidemiologic intentions, 4. rapid detection of resistance to antibiotics.
Keywords
Bacterial Infections/genetics, Bacterial Infections/microbiology, Bacterial Toxins/genetics, DNA Probes, Drug Resistance, Microbial/genetics, Humans, Mycobacterium/classification, Mycobacterium/genetics, Mycobacterium tuberculosis/genetics, Polymerase Chain Reaction
Pubmed
Create date
11/02/2008 13:40
Last modification date
20/08/2019 16:57
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