Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study.

Details

Serval ID
serval:BIB_D7A349224104
Type
Article: article from journal or magazin.
Collection
Publications
Title
Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study.
Journal
Journal of Electron Microscopy Technique
Author(s)
Stierhof Y.D., Humbel B.M., Schwarz H.
ISSN
1059-910X
ISSN-L
0741-0581
Publication state
Published
Issued date
1991
Volume
17
Number
3
Pages
336-343
Language
english
Abstract
In order to exploit the recently introduced 1 nm gold colloids in routine electron microscopic labeling experiments, an efficient enhancement step for a better visualization of this small marker is a prerequisite. Efficiency and reproducibility of enhancement as well as growth homogeneity of gold particles were evaluated for three different silver intensifying solutions: silver lactate/hydroquinone/gum arabic (Danscher, 1981), Ilford L4/Metol (Bienz et al., 1986), and the commercially available IntenSE M silver enhancement kit (Janssen Pharmaceutica). The best results were obtained by using the silver lactate/hydroquinone/gum arabic mixture. The quality of enhancement of the IntenSE M kit was considerably increased by the addition of the protective colloid gum arabic.
Keywords
Bacterial Outer Membrane Proteins/analysis, Escherichia coli/ultrastructure, Gold Colloid, Radioactive, Gum Arabic, Hydroquinones, Lactates, Lactic Acid, Microscopy, Immunoelectron, Silver
Pubmed
Web of science
Create date
18/10/2012 13:09
Last modification date
20/08/2019 15:57
Usage data