Dextramers: new generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells.

Details

Serval ID
serval:BIB_D79B52C290F3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Dextramers: new generation of fluorescent MHC class I/peptide multimers for visualization of antigen-specific CD8+ T cells.
Journal
Journal of immunological methods
Author(s)
Batard P., Peterson D.A., Devêvre E., Guillaume P., Cerottini J.C., Rimoldi D., Speiser D.E., Winther L., Romero P.
ISSN
0022-1759
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
310
Number
1-2
Pages
136-148
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Direct identification as well as isolation of antigen-specific T cells became possible since the development of "tetramers" based on avidin-fluorochrome conjugates associated with mono-biotinylated class I MHC-peptide monomeric complexes. In principle, a series of distinct class I MHC-peptide tetramers, each labelled with a different fluorochrome, would allow to simultaneously enumerate as many unique antigen-specific CD8(+) T cells. Practically, however, only phycoerythrin and allophycocyanin conjugated tetramers have been generally available, imposing serious constraints for multiple labeling. To overcome this limitation, we have developed dextramers which are multimers based on a dextran backbone bearing multiple fluorescein and streptavidin moieties. Here we demonstrate the functionality and optimization of these new probes on human CD8(+) T cell clones with four independent antigen specificities. Their applications to the analysis of relatively low frequency antigen-specific T cells in peripheral blood, as well as their use in fluorescence microscopy, are demonstrated. The data show that dextramers produce a stronger signal than their fluoresceinated tetramer counterparts. Thus, these could become the reagents of choice as the antigen-specific T cell labeling transitions from basic research to clinical application.
Keywords
Antigens, Neoplasm, CD8-Positive T-Lymphocytes/immunology, Dextrans/chemistry, Dextrans/immunology, Epitopes, T-Lymphocyte/immunology, Flow Cytometry, Fluorescent Dyes/chemistry, HLA-A2 Antigen/chemistry, HLA-A2 Antigen/immunology, Humans, Microscopy, Fluorescence, Molecular Weight, Neoplasm Proteins/chemistry, Neoplasm Proteins/immunology, Phosphoproteins/chemistry, Phosphoproteins/immunology, T-Lymphocytes, Cytotoxic/immunology, Trans-Activators/chemistry, Trans-Activators/immunology, Viral Matrix Proteins/chemistry, Viral Matrix Proteins/immunology, Viral Proteins/chemistry, Viral Proteins/immunology
Pubmed
Web of science
Create date
28/01/2008 11:13
Last modification date
20/08/2019 15:57
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