Optimal culture incubation time in orthopedic device-associated infections: a retrospective analysis of prolonged 14-day incubation.

Details

Serval ID
serval:BIB_D77F750AC340
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Optimal culture incubation time in orthopedic device-associated infections: a retrospective analysis of prolonged 14-day incubation.
Journal
Journal of Clinical Microbiology
Author(s)
Schwotzer N., Wahl P., Fracheboud D., Gautier E., Chuard C.
ISSN
1098-660X (Electronic)
ISSN-L
0095-1137
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
52
Number
1
Pages
61-66
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Accurate diagnosis of orthopedic device-associated infections can be challenging. Culture of tissue biopsy specimens is often considered the gold standard; however, there is currently no consensus on the ideal incubation time for specimens. The aim of our study was to assess the yield of a 14-day incubation protocol for tissue biopsy specimens from revision surgery (joint replacements and internal fixation devices) in a general orthopedic and trauma surgery setting. Medical records were reviewed retrospectively in order to identify cases of infection according to predefined diagnostic criteria. From August 2009 to March 2012, 499 tissue biopsy specimens were sampled from 117 cases. In 70 cases (59.8%), at least one sample showed microbiological growth. Among them, 58 cases (82.9%) were considered infections and 12 cases (17.1%) were classified as contaminations. The median time to positivity in the cases of infection was 1 day (range, 1 to 10 days), compared to 6 days (range, 1 to 11 days) in the cases of contamination (P < 0.001). Fifty-six (96.6%) of the infection cases were diagnosed within 7 days of incubation. In conclusion, the results of our study show that the incubation of tissue biopsy specimens beyond 7 days is not productive in a general orthopedic and trauma surgery setting. Prolonged 14-day incubation might be of interest in particular situations, however, in which the prevalence of slow-growing microorganisms and anaerobes is higher.
Pubmed
Web of science
Create date
07/02/2014 18:41
Last modification date
20/08/2019 15:57
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