Establishment and characterization of models of chemotherapy resistance in colorectal cancer: Towards a predictive signature of chemoresistance.

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State: Public
Version: Final published version
Serval ID
serval:BIB_D760B1D2E7A7
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Establishment and characterization of models of chemotherapy resistance in colorectal cancer: Towards a predictive signature of chemoresistance.
Journal
Molecular Oncology
Author(s)
Jensen N.F., Stenvang J., Beck M.K., Hanáková B., Belling K.C., Do K.N., Viuff B., Nygård S.B., Gupta R., Rasmussen M.H., Tarpgaard L.S., Hansen T.P., Budinská E., Pfeiffer P., Bosman F., Tejpar S., Roth A., Delorenzi M., Andersen C.L., Rømer M.U., Brünner N., Moreira J.M.
ISSN
1878-0261 (Electronic)
ISSN-L
1574-7891
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
9
Number
6
Pages
1169-1185
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Current standard treatments for metastatic colorectal cancer (CRC) are based on combination regimens with one of the two chemotherapeutic drugs, irinotecan or oxaliplatin. However, drug resistance frequently limits the clinical efficacy of these therapies. In order to gain new insights into mechanisms associated with chemoresistance, and departing from three distinct CRC cell models, we generated a panel of human colorectal cancer cell lines with acquired resistance to either oxaliplatin or irinotecan. We characterized the resistant cell line variants with regards to their drug resistance profile and transcriptome, and matched our results with datasets generated from relevant clinical material to derive putative resistance biomarkers. We found that the chemoresistant cell line variants had distinctive irinotecan- or oxaliplatin-specific resistance profiles, with non-reciprocal cross-resistance. Furthermore, we could identify several new, as well as some previously described, drug resistance-associated genes for each resistant cell line variant. Each chemoresistant cell line variant acquired a unique set of changes that may represent distinct functional subtypes of chemotherapy resistance. In addition, and given the potential implications for selection of subsequent treatment, we also performed an exploratory analysis, in relevant patient cohorts, of the predictive value of each of the specific genes identified in our cellular models.
Pubmed
Web of science
Open Access
Yes
Create date
12/03/2015 13:04
Last modification date
20/08/2019 15:57
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