Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.

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Serval ID
serval:BIB_D750C0123445
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.
Journal
Nature communications
Author(s)
Botte M., Ni D., Schenck S., Zimmermann I., Chami M., Bocquet N., Egloff P., Bucher D., Trabuco M., Cheng RKY, Brunner J.D., Seeger M.A., Stahlberg H., Hennig M.
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
05/04/2022
Peer-reviewed
Oui
Volume
13
Number
1
Pages
1826
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Abstract
Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria. The last step of LPS insertion via the Lpt pathway is mediated by the LptD/E protein complex. Detailed insights into the architecture of LptDE transporter complexes have been derived from X-ray crystallography. However, no structure of a laterally open LptD transporter, a transient state that occurs during LPS release, is available to date. Here, we report a cryo-EM structure of a partially opened LptDE transporter in complex with rigid chaperones derived from nanobodies, at 3.4 Å resolution. In addition, a subset of particles allows to model a structure of a laterally fully opened LptDE complex. Our work offers insights into the mechanism of LPS insertion, provides a structural framework for the development of antibiotics targeting LptD and describes a highly rigid chaperone scaffold to enable structural biology of challenging protein targets.
Keywords
Bacterial Outer Membrane Proteins/metabolism, Biological Transport, Cryoelectron Microscopy, Crystallography, X-Ray, Escherichia coli Proteins/metabolism, Gram-Negative Bacteria/metabolism, Lipopolysaccharides/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
09/06/2023 16:02
Last modification date
25/01/2024 8:45
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