Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.
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Version: Final published version
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State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_D750C0123445
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryo-EM structures of a LptDE transporter in complex with Pro-macrobodies offer insight into lipopolysaccharide translocation.
Journal
Nature communications
ISSN
2041-1723 (Electronic)
ISSN-L
2041-1723
Publication state
Published
Issued date
05/04/2022
Peer-reviewed
Oui
Volume
13
Number
1
Pages
1826
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: epublish
Publication Status: epublish
Abstract
Lipopolysaccharides are major constituents of the extracellular leaflet in the bacterial outer membrane and form an effective physical barrier for environmental threats and for antibiotics in Gram-negative bacteria. The last step of LPS insertion via the Lpt pathway is mediated by the LptD/E protein complex. Detailed insights into the architecture of LptDE transporter complexes have been derived from X-ray crystallography. However, no structure of a laterally open LptD transporter, a transient state that occurs during LPS release, is available to date. Here, we report a cryo-EM structure of a partially opened LptDE transporter in complex with rigid chaperones derived from nanobodies, at 3.4 Å resolution. In addition, a subset of particles allows to model a structure of a laterally fully opened LptDE complex. Our work offers insights into the mechanism of LPS insertion, provides a structural framework for the development of antibiotics targeting LptD and describes a highly rigid chaperone scaffold to enable structural biology of challenging protein targets.
Keywords
Bacterial Outer Membrane Proteins/metabolism, Biological Transport, Cryoelectron Microscopy, Crystallography, X-Ray, Escherichia coli Proteins/metabolism, Gram-Negative Bacteria/metabolism, Lipopolysaccharides/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
09/06/2023 16:02
Last modification date
25/01/2024 8:45