Photodetection of early human bladder cancer based on the fluorescence of 5-aminolaevulinic acid hexylester-induced protoporphyrin IX: a pilot study.

Details

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State: Public
Version: Final published version
Serval ID
serval:BIB_D6EC551B602E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Photodetection of early human bladder cancer based on the fluorescence of 5-aminolaevulinic acid hexylester-induced protoporphyrin IX: a pilot study.
Journal
British journal of cancer
Author(s)
Lange N., Jichlinski P., Zellweger M., Forrer M., Marti A., Guillou L., Kucera P., Wagnières G., van den Bergh H.
ISSN
0007-0920 (Print)
ISSN-L
0007-0920
Publication state
Published
Issued date
04/1999
Peer-reviewed
Oui
Volume
80
Number
1-2
Pages
185-193
Language
english
Notes
Publication types: Clinical Trial ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Exogenous administration of 5-aminolaevulinic acid (ALA) is becoming widely used to enhance the endogenous synthesis of protoporphyrin IX (PpIX) in photodynamic therapy (PDT) and fluorescence photodetection (PD). Recently, results have shown that the chemical modification of ALA into its more lipophilic esters circumvents limitations of ALA-induced PpIX like shallow penetration depth into deep tissue layers and inhomogeneous biodistribution and enhances the total PpIX formation. The present clinical pilot study assesses the feasibility and the advantages of a topical ALA ester-based fluorescence photodetection in the human bladder. In this preliminary study 5-aminolaevulinic acid hexylester (h-ALA) solutions, containing concentrations ranging from 4 to 16 mM, were applied intravesically to 25 patients. Effects of time and drug dose on the resulting PpIX fluorescence level were determined in vivo with an optical fibre-based spectrofluorometer. Neither local nor systemic side-effects were observed for the applied conditions. All conditions used yielded a preferential PpIX accumulation in the neoplastic tissue. Our clinical investigations indicate that with h-ALA a twofold increase of PpIX fluorescence intensity can be observed using 20-fold lower concentrations as compared to ALA.

Keywords
Aged, Aminolevulinic Acid/administration & dosage, Aminolevulinic Acid/pharmacokinetics, Female, Fluorescence, Humans, Male, Middle Aged, Photochemotherapy, Photosensitizing Agents/administration & dosage, Photosensitizing Agents/pharmacokinetics, Pilot Projects, Protoporphyrins/administration & dosage, Protoporphyrins/pharmacokinetics, Spectrometry, Fluorescence/instrumentation, Urinary Bladder Neoplasms/diagnosis, Urinary Bladder Neoplasms/metabolism, Urinary Bladder Neoplasms/pathology, Urinary Bladder Neoplasms/therapy
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:09
Last modification date
20/08/2019 15:56
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