Prejunctional angiotensin II receptors. Facilitation of norepinephrine release in the human forearm

Details

Serval ID
serval:BIB_D6E9B2AF8C32
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Prejunctional angiotensin II receptors. Facilitation of norepinephrine release in the human forearm
Journal
Journal of Clinical Investigation
Author(s)
Clemson  B., Gaul  L., Gubin  S. S., Campsey  D. M., McConville  J., Nussberger  J., Zelis  R.
ISSN
0021-9738 (Print)
Publication state
Published
Issued date
02/1994
Volume
93
Number
2
Pages
684-91
Notes
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Feb
Abstract
To determine if peripheral angiotensin II (Ang II) prejunctional receptors facilitating NE release exist in humans, we used [3H]NE kinetic methodology to measure forearm NE spillover during intrabrachial arterial Ang II infusions in eight normal male subjects. We used the following protocol to optimize conditions for demonstrating these receptors: (a) lower body negative pressure (-15 mmHg) to increase sympathetic nerve activity to skeletal muscle; and (b) intraarterial nitroprusside to maintain a high constant forearm blood flow (approximately 10 ml/min.100 ml) to maximize the proportion of neuronally released NE that spills over into the circulation. During lower body negative pressure, the following were infused intraarterially for three consecutive 20-min periods: saline, Ang II (4 ng/min), and Ang II (16 ng/min). During the Ang II infusions, forearm venous NE increased significantly from 173 to 189 and 224 pg/ml (P < 0.01), and forearm NE spillover increased from 384 to 439 and 560 ng/min.100 ml (P < 0.05 for high Ang II). Forearm NE clearance was unchanged. During low and high dose Ang II, the plasma venous Ang II concentrations were 25 and 97 pM, respectively. Since normal subjects increase plasma Ang II from 4 to 20-22 pM with exercise, standing, or diuretic administration, and patients with severe congestive heart failure can have a plasma Ang II of approximately 25 pM at rest, we suggest that Ang II might facilitate NE release in severe congestive heart failure, especially under conditions of stress.
Keywords
Adult Angiotensin II/administration & dosage/*pharmacology Arteries Blood Pressure/drug effects Chromatography, High Pressure Liquid Forearm/blood supply Heart Rate/drug effects Humans Infusions, Intra-Arterial Male Muscles/blood supply/innervation/*physiology Norepinephrine/blood/*secretion Receptors, Angiotensin/drug effects/*physiology Regional Blood Flow Veins
Pubmed
Web of science
Open Access
Yes
Create date
05/03/2008 16:39
Last modification date
20/08/2019 15:56
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