miR-210 links hypoxia with cell cycle regulation and is deleted in human epithelial ovarian cancer.

Details

Serval ID
serval:BIB_D6D247259F8A
Type
Article: article from journal or magazin.
Collection
Publications
Title
miR-210 links hypoxia with cell cycle regulation and is deleted in human epithelial ovarian cancer.
Journal
Cancer Biology and Therapy
Author(s)
Giannakakis A., Sandaltzopoulos R., Greshock J., Liang S., Huang J., Hasegawa K., Li C., O'Brien-Jenkins A., Katsaros D., Weber B.L., Simon C., Coukos G., Zhang L.
ISSN
1555-8576 (Electronic)
ISSN-L
1538-4047
Publication state
Published
Issued date
2008
Volume
7
Number
2
Pages
255-264
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Abstract
Tumor growth results in hypoxia. Understanding the mechanisms of gene expression reprogramming under hypoxia may provide important clues to cancer pathogenesis. We studied miRNA genes that are regulated by hypoxia in ovarian cancer cell lines by TaqMan miRNA assay containing 157 mature miRNAs. MiR-210 was the most prominent miRNA consistently stimulated under hypoxic conditions. We provide evidence for the involvement of the HIF signaling pathway in miR-210 regulation. Biocomputational analysis and in vitro assays demonstrated that e2f transcription factor 3 (e2f3), a key protein in cell cycle, is regulated by miR-210. E2F3 was further confirmed to be downregulated at the protein level upon induction of miR-210. Importantly, we found remarkably high frequency of miR-210 gene copy deletions in ovarian cancer patients (64%, n = 114) and that gene copy number correlates with miR-210 expression levels. Taken together, our results indicate that miR-210 plays a crucial role in tumor onset as a key regulator of the hypoxia response and provide evidence for a link between hypoxia and the regulation of cell cycle.
Keywords
Anoxia/metabolism, Cell Cycle/physiology, Cell Line, Tumor, Computational Biology/methods, Female, Gene Dosage, Gene Expression Regulation, Neoplastic, Humans, MicroRNAs/genetics, MicroRNAs/metabolism, Ovarian Neoplasms/genetics, Ovarian Neoplasms/metabolism, Sequence Deletion
Pubmed
Web of science
Create date
14/10/2014 11:43
Last modification date
20/08/2019 15:56
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