Ex vivo monitoring of antigen-specific CD4+ T cells after recall immunization with tetanus toxoid

Details

Serval ID
serval:BIB_D6C3E5C6DC51
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Ex vivo monitoring of antigen-specific CD4+ T cells after recall immunization with tetanus toxoid
Journal
Clinical and Vaccine Immunology
Author(s)
Barbey C., Pradervand E., Barbier N., Spertini F.
ISSN
1556-6811 (Print)
Publication state
Published
Issued date
2007
Volume
14
Number
9
Pages
1108-16
Notes
Journal Article Research Support, Non-U.S. Gov't
Abstract
To monitor antigen-specific CD4+ T cells during a recall immune response to tetanus toxoid (TT), a sequential analysis including ex vivo phenotyping and cytokine flow cytometry, followed by cloning and T-cell-receptor (TCR) spectratyping of cytokine-positive CD4+ T cells, was performed. Grossly, twice as many TT-specific CD4+ T-cell clones, ex vivo derived from the CCR7+/- CD69+ interleukin-2-positive (IL-2+) CD4+ subsets, belonged to the central memory (T(CM); CD62L+ CD27+ CCR7+) compared to the effector memory population (T(EM); CD62L- CD27- CCR7-). After the boost, a predominant expansion of the T(CM) population was observed with more limited variations of the T(EM) population. TCR beta-chain-variable region (BV) spectratyping and sequencing confirmed a large concordance between most frequently expressed BV TCR-CDR3 from ex vivo-sorted CCR7+/- CD69+ IL-2+ CD4+ subsets and BV usage of in vitro-derived TT-specific CD4+ T-cell clones, further demonstrating the highly polyclonal but stable character of the specific recall response to TT. Taken together, ex vivo flow cytometry analysis focused on the CCR7+/- CD69+ IL-2+ CD4+ subsets appears to target the bulk of antigen-specific T cells and to reach an analytical power sufficient to adequately delineate in field trials the profile of the antigen-specific response to vaccine.
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 15:19
Last modification date
20/08/2019 15:56
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