Development of novel broad-range pan-genus PCR assays for the detection of Tropheryma species.
Details
Serval ID
serval:BIB_D6ABCC5D8FB1
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Development of novel broad-range pan-genus PCR assays for the detection of Tropheryma species.
Journal
Journal of medical microbiology
ISSN
1473-5644 (Electronic)
ISSN-L
0022-2615
Publication state
Published
Issued date
09/2024
Peer-reviewed
Oui
Volume
73
Number
9
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Introduction. Tropheryma whipplei is responsible for the classical Whipple's disease. Recently, a new Tropheryma species was described in a Belgian immunocompromised patient with pleuritis.Gap Statement. There is currently no specific molecular diagnostic test detecting other Tropheryma species than Tropheryma whipplei.Aim. To develop and validate two broad-range pan-Tropheryma genus PCRs detecting both T. whipplei and new Tropheryma species.Methodology. From shotgun sequencing data of the lung tissue biopsy of the Belgian subject, we designed two PCRs targeting the 23S rRNA and rnpB genes. Prospectively, requests for T. whipplei PCR were tested with T. whipplei-specific PCRs and the two Tropheryma broad-range PCRs from January 2019 to November 2022.Results. In total, 2605 samples were tested using both the pan-Tropheryma 23S rRNA PCR and the T. whipplei-specific PCR. In addition, 833 of the 2605 samples were also tested using the pan-Tropheryma rnpB PCRs. Sensitivity was 78.8% and 79.7% for 23S rRNA and rnpB PCRs, as compared with the species-specific T. whipplei PCR. Specificity was 99.9% and 99.7% for the 23S rRNA and the rnpB PCRs, respectively. We identified a patient whose bronchoalveolar lavage tested positive with the two broad-range PCRs with >10 <sup>5</sup> copies ml <sup>-1</sup> . Specific T. whipplei PCRs were negative. Known for panuveitis, this 49-year-old male presented with an eye inflammation recurrence, and a CT scan showed multiple mediastino-hilar necrotic adenopathies. Doxycyclin (1 year), hydroxychloroquin (1 year) and co-trimoxazol (1 month) treatments led to a favourable outcome.Conclusion. Specific T. whipplei PCR exhibited better sensitivity than the pan-Tropheryma PCRs. However, both broad-range pan-Tropheryma PCRs demonstrated excellent specificity and were pivotal to identifying a new probable case of Tropheryma infection due to another species-level lineage.
Keywords
Humans, Tropheryma/genetics, Tropheryma/isolation & purification, Polymerase Chain Reaction/methods, RNA, Ribosomal, 23S/genetics, Sensitivity and Specificity, Whipple Disease/diagnosis, Whipple Disease/microbiology, DNA, Bacterial/genetics, Belgium, Molecular Diagnostic Techniques/methods, Lung/microbiology, Lung/pathology, Male, 16S rRNA, colonization, lymphadenopathy, molecular diagnosis, pneumopathy, whole-genome sequencing
Pubmed
Create date
27/09/2024 15:58
Last modification date
28/09/2024 7:09