Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candida albicans strains.

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Serval ID
serval:BIB_D64914E58A71
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Rhodamine 6G efflux for the detection of CDR1-overexpressing azole-resistant Candida albicans strains.
Journal
The Journal of Antimicrobial Chemotherapy
Author(s)
Maesaki S., Marichal P., Vanden Bossche H., Sanglard D., Kohno S.
ISSN
0305-7453
Publication state
Published
Issued date
07/1999
Peer-reviewed
Oui
Volume
44
Number
1
Pages
27-31
Language
english
Notes
Publication types: Journal Article
Abstract
We investigated the drug efflux mechanism in azole-resistant strains of Candida albicans using rhodamine 6G (R6G). No significant differences in R6G uptake were observed between azole-sensitive B2630 (9.02 +/- 0.02 nmol/10(8) cells) and azole-resistant B67081 (8.86 +/- 0.03 nmol/10(8) cells) strains incubated in glucose-free phosphate buffered saline. A significantly higher R6G efflux (2.0 +/- 0.21 nmol/10(8) cells) was noted in the azole-resistant strain (B67081) when glucose was added, compared with that in the sensitive strain B2630 (0.23 < or = 0.14 nmol/10(8) cells). A fluconazole-resistant strain C40 that expressed the benomyl resistance gene (CaMDR) also showed a low R6G efflux (0.16 +/- 0.06 nmol/10(8) cells) as did the sensitive strains. Accumulation of R6G in growing C. albicans cells was inversely correlated with the level of CDR1 mRNA expression. Our data also suggest that measurement of intracellular accumulation of R6G is a useful method for identification of azole-resistant strains due to CDR1-expressed drug efflux pump.
Keywords
Antifungal Agents/pharmacology, Azoles/pharmacology, Benomyl/pharmacology, Candida albicans/drug effects, Candida albicans/genetics, Coloring Agents/metabolism, Drug Resistance, Microbial/genetics, Fungal Proteins/biosynthesis, Fungal Proteins/genetics, Genes, MDR, Humans, Membrane Transport Proteins, Microbial Sensitivity Tests, Rhodamines/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 14:40
Last modification date
25/09/2019 6:10
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