Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates.

Details

Serval ID
serval:BIB_D5E19B77645B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Transcriptome Analysis of Pseudomonas aeruginosa Cultured in Human Burn Wound Exudates.
Journal
Frontiers in cellular and infection microbiology
Author(s)
Gonzalez M.R., Ducret V., Leoni S., Fleuchot B., Jafari P., Raffoul W., Applegate L.A., Que Y.A., Perron K.
ISSN
2235-2988 (Electronic)
ISSN-L
2235-2988
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
8
Pages
39
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
javax.xml.bind.JAXBElement@7c3898e
is a severe opportunistic pathogen and is one of the major causes of hard to treat burn wound infections. Herein we have used an RNA-seq transcriptomic approach to study the behavior of javax.xml.bind.JAXBElement@19de7c8c PAO1 growing directly on human burn wound exudate. A chemical analysis of compounds used by this bacterium, coupled with kinetics expression of central genes has allowed us to obtain a global view of javax.xml.bind.JAXBElement@32725f17 physiological and metabolic changes occurring while growing on human burn wound exudate. In addition to the numerous virulence factors and their secretion systems, we have found that all iron acquisition mechanisms were overexpressed. Deletion and complementation with pyoverdine demonstrated that iron availability was a major limiting factor in burn wound exudate. The quorum sensing systems, known to be important for the virulence of javax.xml.bind.JAXBElement@4d315003 , although moderately induced, were activated even at low cell density. Analysis of bacterial metabolism emphasized importance of lactate, lipid and collagen degradation pathways. Overall, this work allowed to designate, for the first time, a global view of javax.xml.bind.JAXBElement@56a53fd2 characteristics while growing in human burn wound exudate and highlight the possible therapeutic approaches to combat javax.xml.bind.JAXBElement@3cf50396 burn wound infections.

Keywords
Pseudomonas aeruginosa, human burn wound exudates, infection, transcriptome, virulence factors
Pubmed
Web of science
Open Access
Yes
Create date
15/03/2018 18:38
Last modification date
20/08/2019 16:55
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