A novel function of RNAs arising from the long terminal repeat of human endogenous retrovirus 9 in cell cycle arrest

Details

Serval ID
serval:BIB_D59B85B6906D
Type
Article: article from journal or magazin.
Collection
Publications
Title
A novel function of RNAs arising from the long terminal repeat of human endogenous retrovirus 9 in cell cycle arrest
Journal
J Virol
Author(s)
Xu L., Elkahloun A. G., Candotti F., Grajkowski A., Beaucage S. L., Petricoin E. F., Calvert V., Juhl H., Mills F., Mason K., Shastri N., Chik J., Xu C., Rosenberg A. S.
ISSN
1098-5514 (Electronic)
ISSN-L
0022-538X
Publication state
Published
Issued date
01/2013
Volume
87
Number
1
Pages
25-36
Language
english
Notes
Xu, Lai
Elkahloun, Abdel G
Candotti, Fabio
Grajkowski, Andrzej
Beaucage, Serge L
Petricoin, Emanuel F
Calvert, Valerie
Juhl, Hartmut
Mills, Frederick
Mason, Karen
Shastri, Neal
Chik, Josh
Xu, Cynthia
Rosenberg, Amy S
eng
Intramural NIH HHS/
Research Support, N.I.H., Intramural
J Virol. 2013 Jan;87(1):25-36. doi: 10.1128/JVI.01648-12. Epub 2012 Oct 24.
Abstract
The human genome contains approximately 50 copies of the replication-defective human endogenous retrovirus 9 (ERV-9) and thousands of copies of its solitary long term repeat (sLTR) element. While some sLTRs are located upstream of critical genes and have enhancer activity, other sLTRs are located within introns and may be transcribed as RNAs. We found that intronic RNAs arising from U3 sLTRs of ERV-9 were expressed as both sense (S) and antisense (AS) transcripts in all human cells tested but that expression levels differed in malignant versus nonmalignant cells. In nonmalignant cells, AS was expressed at higher levels than S and at higher levels than in malignant cells; in malignant cells, AS was expressed at amounts equivalent to those of S RNA. Critically, U3 AS RNA was found to physically bind to key transcription factors for cellular proliferation, including NF-Y, p53, and sp1, indicating that such RNA transcripts may function as decoy targets or traps for NF-Y and thus inhibit the growth of human cancer cells. Indeed, short U3 oligodeoxynucleotides (ODNs) based on these RNA sequences ably inhibited proliferation of cancer cell lines driven by cyclins B1/B2, the gene targets of NF-Y.
Keywords
*Cell Cycle Checkpoints, Cell Line, Tumor, Endogenous Retroviruses/*pathogenicity, Humans, Protein Binding, RNA, Antisense/*biosynthesis/genetics, RNA, Viral/*biosynthesis/genetics, Terminal Repeat Sequences/*genetics, Transcription Factors/metabolism, *Transcription, Genetic
Pubmed
Open Access
Yes
Create date
01/11/2017 10:29
Last modification date
20/08/2019 15:55
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