Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.

Details

Serval ID
serval:BIB_D5727ACDC88B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of double-locus sequence typing (DLST) and multilocus sequence typing (MLST) for the investigation of Pseudomonas aeruginosa populations.
Journal
Diagnostic Microbiology and Infectious Disease
Author(s)
Cholley P., Stojanov M., Hocquet D., Thouverez M., Bertrand X., Blanc D.S.
ISSN
1879-0070 (Electronic)
ISSN-L
0732-8893
Publication state
Published
Issued date
2015
Peer-reviewed
Oui
Volume
82
Number
4
Pages
274-277
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
Reliable molecular typing methods are necessary to investigate the epidemiology of bacterial pathogens. Reference methods such as multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) are costly and time consuming. Here, we compared our newly developed double-locus sequence typing (DLST) method for Pseudomonas aeruginosa to MLST and PFGE on a collection of 281 isolates. DLST was as discriminatory as MLST and was able to recognize "high-risk" epidemic clones. Both methods were highly congruent. Not surprisingly, a higher discriminatory power was observed with PFGE. In conclusion, being a simple method (single-strand sequencing of only 2 loci), DLST is valuable as a first-line typing tool for epidemiological investigations of P. aeruginosa. Coupled to a more discriminant method like PFGE or whole genome sequencing, it might represent an efficient typing strategy to investigate or prevent outbreaks.
Pubmed
Web of science
Create date
13/07/2015 10:01
Last modification date
20/08/2019 16:55
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