CD8+ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria

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Serval ID
serval:BIB_D54952DB1643
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
CD8+ cytolytic T cell clones derived against the Plasmodium yoelii circumsporozoite protein protect against malaria
Journal
International Immunology
Author(s)
Rodrigues  M. M., Cordey  A. S., Arreaza  G., Corradin  G., Romero  P., Maryanski  J. L., Nussenzweig  R. S., Zavala  F.
ISSN
0953-8178 (Print)
Publication state
Published
Issued date
06/1991
Volume
3
Number
6
Pages
579-85
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jun
Abstract
Immunization of BALB/c mice with radiation-attenuated Plasmodium yoelii sporozoites induces cytotoxic T lymphocytes (CTL) specific for an epitope located within the amino acid sequence 277-288 of the P. yoelii circumsporozoite (CS) protein. Several CD8+ CTL clones were derived from the spleen cells of sporozoite-immunized mice, all displaying an apparently identical epitope specificity. All the clones induced high levels of cytolysis in vitro upon exposure to peptide-incubated MHC-compatible target cells. The adoptive transfer of two of these clones conferred complete protection against sporozoite challenge to naive mice. This protection is species and stage specific. Using P. yoelii specific ribosomal RNA probes to monitor the in vivo effects of the CTL clones, we found that their target was the intrahepatocytic stage of the parasite. The protective clones completely inhibited the development of the liver stages of P. yoelii. Some CTL clones were only partially inhibitory in vivo, while others failed completely to alter liver stage development and to confer any detectable degree of protection. The elucidation of the effector mechanism of this CTL mediated protection against rodent malaria should facilitate the design of an effective malaria vaccine. From a broader perspective this model may provide further insight into the mechanism(s) of CTL mediated killing of intracellular non-viral pathogens in general.
Keywords
Animals Antigens, CD8 Antigens, Differentiation, T-Lymphocyte Antigens, Protozoan/*immunology Epitopes Female Immunotherapy, Adoptive Interferon Type II/antagonists & inhibitors Malaria/immunology/*prevention & control Mice Mice, Inbred BALB C Plasmodium berghei/immunology Plasmodium yoelii/growth & development/*immunology *Protozoan Proteins Species Specificity T-Lymphocytes, Cytotoxic/*immunology Tumor Necrosis Factor-alpha/antagonists & inhibitors
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:28
Last modification date
01/10/2019 7:19
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