New anti-GD2 monoclonal antibodies produced from gamma-interferon-treated neuroblastoma cells

Details

Serval ID
serval:BIB_D490F9C7943A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
New anti-GD2 monoclonal antibodies produced from gamma-interferon-treated neuroblastoma cells
Journal
International Journal of Cancer
Author(s)
Gross  N., Beck  D., Portoukalian  J., Favre  S., Carrel  S.
ISSN
0020-7136
Publication state
Published
Issued date
04/1989
Peer-reviewed
Oui
Volume
43
Number
4
Pages
665-71
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Apr 15
Abstract
Three monoclonal antibodies (IgG2) have been produced from hybridomas obtained by fusion of murine myeloma cells and spleen cells of mice hyperimmunized with gamma-interferon-treated neuroblastoma cells. The 3 MAbs, 7A4, 2A6 and IG8, detected an antigen present on neuroblastoma tumors and cell lines, but also on some neuro-ectoderm-derived tissues and cells. All 3 clones were shown to react with an epitope of the di-sialo-ganglioside GD2 molecules highly expressed by some neuro-ectoderm-derived tumors, mainly neuroblastoma. Whereas MAb IG8 specificity was restricted to GD2 and its o-acylated form, MAb 2A6 and 7A4 were also able to detect GD3 at high concentration of antibody as shown by TLC analysis and immunodetection. The 3 MAbs were able to lyse 100% neuroblastoma cells in the presence of rabbit or human complement. Direct binding assays with 125I-labelled MAbs showed that MAb 7A4 might be a good candidate for in vivo immunolocalization experiments. The high proportion of anti-GD2 MAbs obtained by our fusion and the increased binding of anti-GD2 MAbs on gamma-IFN-treated neuroblastoma cells suggests a modulation of the exposure and an increase in the immunogenicity of GD2 induced by gamma-IFN.
Keywords
Animals Antibodies, Monoclonal/analysis/*biosynthesis/isolation & purification Antigens, Neoplasm/analysis Binding Sites, Antibody/drug effects Cell Line Complement System Proteins/immunology Cytotoxicity, Immunologic Epitopes/analysis/immunology Gangliosides/*immunology/isolation & purification Humans Hybridomas/drug effects/immunology Immunization/methods Immunoglobulin Isotypes/analysis Interferon Type II/*pharmacology Mice Neuroblastoma/*immunology
Pubmed
Web of science
Create date
20/01/2008 15:55
Last modification date
20/08/2019 15:54
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