Silent infection of human dendritic cells by African and Asian strains of Zika virus.

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License: CC BY 4.0
Serval ID
serval:BIB_D48377936062
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Silent infection of human dendritic cells by African and Asian strains of Zika virus.
Journal
Scientific reports
Author(s)
Vielle N.J., Zumkehr B., García-Nicolás O., Blank F., Stojanov M., Musso D., Baud D., Summerfield A., Alves M.P.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
03/04/2018
Peer-reviewed
Oui
Volume
8
Number
1
Pages
5440
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
While Zika virus (ZIKV) circulated for decades (African lineage strains) without report of outbreaks and severe complications, its emergence in French Polynesia and subsequently in the Americas (Asian lineage strains) was associated with description of severe neurological defects in newborns/neonates and adults. With the aim to identify virus lineage-dependent factors, we compared cell susceptibility, virus replication, cell death and innate immune responses following infection with two African and three contemporary Asian lineage strains of ZIKV. To this end, we used green monkey Vero and Aedes albopictus C6/36 cells and human monocyte-derived dendritic cells (DCs). The latter are involved in the pathogenesis of several mosquito-borne Flavivirus infections. In Vero and C6/36 cells, we observed strain- but not lineage-dependent differences in infection profiles. Nevertheless, in human DCs, no significant differences in susceptibility and virus replication were found between lineages and strains. ZIKV induced antiviral interferon type I/III in a limited fashion, with the exception of one African strain. None of the strains induced cell death or DC maturation in terms of MHC II, CD40, CD80/86 or CCR7 expression. Taken together, our data suggest that a large collection of virus isolates needs to be investigated before conclusions on lineage differences can be made.
Keywords
Animals, Cercopithecus aethiops, Dendritic Cells/metabolism, Dendritic Cells/virology, Gene Expression Regulation, Humans, Interferons/genetics, Species Specificity, Vero Cells, Zika Virus/physiology
Pubmed
Web of science
Open Access
Yes
Create date
12/04/2018 17:35
Last modification date
30/04/2021 7:15
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