Alginate-coated chitosan nanogel capacity to modulate the effect of TLR ligands on blood dendritic cells.

Details

Serval ID
serval:BIB_D455CE6C35E2
Type
Article: article from journal or magazin.
Collection
Publications
Title
Alginate-coated chitosan nanogel capacity to modulate the effect of TLR ligands on blood dendritic cells.
Journal
Nanomedicine : nanotechnology, biology, and medicine
Author(s)
Démoulins T., Bassi I., Thomann-Harwood L., Jandus C., Kaeuper P., Simon H.U., von Gunten S., McCullough K.C.
ISSN
1549-9642 (Electronic)
ISSN-L
1549-9634
Publication state
Published
Issued date
08/2013
Volume
9
Number
6
Pages
806-817
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Biodegradable nanoparticles have been employed for vaccine delivery, frequently admixed with adjuvants. Surprisingly, there is little information on their modulation of immune responses, speculated to be negligible. We analyzed the immunomodulatory capacity of alginate-coated chitosan nanogels (Ng), on porcine and human blood dendritic cells (DCs), when applied with defined adjuvants targeting different DC subpopulations. DC maturation, cytokine production and cell migration were assessed. Ng differentially influenced the immunomodulatory characteristics of individual Toll-like receptor (TLR) ligands: Pam3Cys-SK4-induced IL-1β was enhanced; CpG-oligodeoxynucleotides (CpG-ODN)-induced IFN-α, IL-6 and TNFα were impaired; CpG-ODN-induced CD86 and CCR7, and cell migration, were diminished-plasmacytoid DCs (pDCs) were particularly sensitive. Therein, the Ng influence on DC endocytosis of the TLR ligands was apparently a major contributory element. This demonstrates the importance of predefining the interplay between delivery vehicles and admixed immunostimulatory moieties, for ensuring appropriate immune activation and efficacious combinations.
Biodegradable nanoparticles have been utilized in vaccine delivery; however, there is little information available on their immunomodulatory properties, which are thought to be negligible. This study clearly demonstrates that nanogels do influence the developing immune response, which needs to be taken into consideration when utilizing these otherwise very efficacious vaccine delivery approaches.

Keywords
Adjuvants, Immunologic/administration & dosage, Alginates/administration & dosage, Alginates/chemistry, Animals, Blood/drug effects, Cell Movement/drug effects, Chitosan/administration & dosage, Chitosan/chemistry, Dendritic Cells/cytology, Dendritic Cells/drug effects, Endocytosis/genetics, Glucuronic Acid/administration & dosage, Glucuronic Acid/chemistry, Hexuronic Acids/administration & dosage, Hexuronic Acids/chemistry, Humans, Ligands, Polyethylene Glycols/administration & dosage, Polyethylene Glycols/chemistry, Polyethyleneimine/administration & dosage, Polyethyleneimine/chemistry, Swine, Toll-Like Receptors/metabolism
Pubmed
Create date
10/02/2017 23:09
Last modification date
20/08/2019 15:54
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