Comparison of random amplified polymorphic DNA with restriction fragment length polymorphism as epidemiological typing methods for Mycobacterium tuberculosis.

Details

Serval ID
serval:BIB_D3D5D605AC35
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Comparison of random amplified polymorphic DNA with restriction fragment length polymorphism as epidemiological typing methods for Mycobacterium tuberculosis.
Journal
Clinical Molecular Pathology
Author(s)
Linton C.J., Smart A.D., Leeming J.P., Jalal H., Telenti A., Bodmer T., Millar M.R.
ISSN
1355-2910 (Print)
ISSN-L
1355-2910
Publication state
Published
Issued date
06/1995
Volume
48
Number
3
Pages
M133-M135
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Aim-To compare restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) methods for the epidemiological typing of Mycobacterium tuberculosis.Methods-Thirty one M tuberculosis cultures originating from patients in the Canton of Berne in Switzerland, which had previously been typed by RFLP, were subjected to RAPD analysis. Cultures were coded so that the investigators were blind to the RFLP results until RAPD analysis was complete.Results-The 31 cultures of M tuberculosis were divided into nine groups by RFLP and eight groups by RAPD. Generally there was good correlation between the groups identified by the two techniques, with the exception of strains that had only one copy of IS6110. Both methods subdivided isolates that were placed in a single group by the other method.Conclusions-RAPD analysis is quick, simple, and useful for the comparison of small numbers of isolates. RFLP is more reproducible and therefore better suited for the accumulation of RFLP fingerprints for long term local surveillance and large epidemiological studies.
Pubmed
Create date
25/01/2008 14:46
Last modification date
20/08/2019 15:53
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