Short periodic applications of the vacuum-assisted closure device cause an extended tissue response in the diabetic mouse model.

Details

Serval ID
serval:BIB_D35A97731FF8
Type
Article: article from journal or magazin.
Collection
Publications
Title
Short periodic applications of the vacuum-assisted closure device cause an extended tissue response in the diabetic mouse model.
Journal
Plastic and reconstructive surgery
Author(s)
Scherer S.S., Pietramaggiori G., Mathews J.C., Orgill D.P.
ISSN
1529-4242 (Electronic)
ISSN-L
0032-1052
Publication state
Published
Issued date
11/2009
Peer-reviewed
Oui
Volume
124
Number
5
Pages
1458-1465
Language
english
Notes
Publication types: Comparative Study ; Journal Article
Publication Status: ppublish
Abstract
The vacuum-assisted closure device is a widely used mechanical modulator of wound healing; however, the optimal time kinetics of application have not been determined. The objective of the study was to optimize the kinetics of vacuum-assisted closure application.
Full-thickness wounds in seven diabetic mice per study group were treated with either an occlusive dressing alone, the vacuum-assisted closure device for 6 or 12 hours, or the vacuum-assisted closure device periodically for 4 hours every other day or continuously for 7 days. Wound closure and tissue response were evaluated by macroscopic, histologic, and immunohistochemical analyses on day 7.
Wound closure was significantly faster after short initial vacuum-assisted closure (6-hour and 12-hour groups) when compared with continuous treatment. Increased granulation tissue formation was seen in the 12-hour group (2.4-fold increase) and in those treated periodically for 4 hours every other day (3.2-fold increase) compared with the dressing-alone controls. Significant stimulation of cell proliferation was seen after all vacuum-assisted closure patterns (3.6- to 5.3-fold increase), whereas angiogenesis was augmented only after the device was applied for either three times for 4 hours (4.3-fold) or continuously (4.7-fold) when compared with dressing-treated wounds. Treatment three times for 4 hours showed a superior angiogenic effect also when compared with short initial applications (6-hour and 12-hour groups).
Short vacuum-assisted closure treatment induced an extended biological response in the wound. A total of 12 hours of periodically applied vacuum-assisted closure reached a similar wound tissue response as continuously applied vacuum-assisted closure for 7 days. These findings suggest new clinical approaches for mechanical wound-healing devices.

Keywords
Animals, Biomarkers/metabolism, Cell Proliferation, Diabetes Mellitus, Experimental, Granulation Tissue/metabolism, Immunohistochemistry, Ki-67 Antigen/metabolism, Kinetics, Mice, Negative-Pressure Wound Therapy/instrumentation, Neovascularization, Physiologic, Occlusive Dressings, Time Factors, Vascular Cell Adhesion Molecule-1/metabolism, Wound Healing/physiology
Pubmed
Web of science
Create date
16/01/2018 15:01
Last modification date
20/08/2019 15:53
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