Deoxyribonucleic acid content as an indicator of progression of squamous cell carcinogenesis in the esophagus: comparative analysis on imprint-cytospin and tissue section preparation.

Details

Serval ID
serval:BIB_D2DD19CAF913
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Deoxyribonucleic acid content as an indicator of progression of squamous cell carcinogenesis in the esophagus: comparative analysis on imprint-cytospin and tissue section preparation.
Journal
Cancer Detection and Prevention
Author(s)
Andrejevic-Blant S., Osterheld M.C., Caron L., Ballini J.P., Monnier P.
ISSN
0361-090X
Publication state
Published
Issued date
2006
Peer-reviewed
Oui
Volume
30
Number
3
Pages
276-283
Language
english
Abstract
BACKGROUND: The purpose of this study was to explore the potential use of image analysis on tissue sections preparation as a predictive marker of early malignant changes during squamous cell (SC) carcinogenesis in the esophagus. Results of DNA ploidy quantification on formalin-fixed, paraffin-embedded tissue using two different techniques were compared: imprint-cytospin and 6 microm thick tissue sections preparation. METHODS: This retrospective study included 26 surgical specimens of squamous cell carcinoma (SCC) from patients who underwent surgery alone at the Department of Surgery in CHUV Hospital in Lausanne between January 1993 and December 2000. We analyzed 53 samples of healthy tissue, 43 tumors and 7 lymph node metastases. RESULTS: Diploid DNA histogram patterns were observed in all histologically healthy tissues, either distant or proximal to the lesion. Aneuploidy was observed in 34 (79%) of 43 carcinomas, namely 24 (75%) of 32 early squamous cell carcinomas and 10 (91%) of 11 advanced carcinomas. DNA content was similar in the different tumor stages, whether patients presented with single or multiple synchronous tumors. All lymph node metastases had similar DNA content as their primary tumor. CONCLUSIONS: Early malignant changes in the esophagus are associated with alteration in DNA content, and aneuploidy tends to correlate with progression of invasive SCC. A very good correlation between imprint-cytospin and tissue section analysis was observed. Although each method used here showed advantages and disadvantages; tissue sections preparation provided useful information on aberrant cell-cycle regulation and helped select the optimal treatment for the individual patient along with consideration of other clinical parameters.
Keywords
Aged, Aged, 80 and over, Carcinoma, Squamous Cell, Cell Transformation, Neoplastic, DNA, DNA, Neoplasm, Disease Progression, Esophageal Neoplasms, Esophagus, Female, Humans, Male, Middle Aged, Neoplasm Metastasis, Neoplasm Staging
Pubmed
Web of science
Create date
29/11/2007 11:33
Last modification date
20/08/2019 15:52
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