Complex formation by the human RAD51C and XRCC3 recombination repair proteins.

Details

Serval ID
serval:BIB_D25FC395A295
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Complex formation by the human RAD51C and XRCC3 recombination repair proteins.
Journal
Proceedings of the National Academy of Sciences of the United States of America
Author(s)
Masson J.Y., Stasiak A.Z., Stasiak A., Benson F.E., West S.C.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Publication state
Published
Issued date
07/2001
Volume
98
Number
15
Pages
8440-8446
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
In vertebrates, the RAD51 protein is required for genetic recombination, DNA repair, and cellular proliferation. Five paralogs of RAD51, known as RAD51B, RAD51C, RAD51D, XRCC2, and XRCC3, have been identified and also shown to be required for recombination and genome stability. At the present time, however, very little is known about their biochemical properties or precise biological functions. As a first step toward understanding the roles of the RAD51 paralogs in recombination, the human RAD51C and XRCC3 proteins were overexpressed and purified from baculovirus-infected insect cells. The two proteins copurify as a complex, a property that reflects their endogenous association observed in HeLa cells. Purified RAD51C--XRCC3 complex binds single-stranded, but not duplex DNA, to form protein--DNA networks that have been visualized by electron microscopy.
Keywords
Animals, Cell Line, DNA Repair, DNA, Single-Stranded/metabolism, DNA-Binding Proteins/isolation & purification, DNA-Binding Proteins/metabolism, HeLa Cells, Humans, Mice, Microscopy, Electron, Oligodeoxyribonucleotides/metabolism, Rabbits, Rad51 Recombinase, Recombinant Fusion Proteins/isolation & purification, Recombinant Fusion Proteins/metabolism, Recombination, Genetic, Spodoptera
Pubmed
Web of science
Create date
24/01/2008 10:36
Last modification date
20/08/2019 15:52
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