Recherche de l'amplification du gène HER2: la CISH est-elle une alternative à la technique FISH [HER2 gene amplification assay: is CISH an alternative to FISH?].

Details

Serval ID
serval:BIB_D1C075A6F195
Type
Article: article from journal or magazin.
Publication sub-type
Review (review): journal as complete as possible of one specific subject, written based on exhaustive analyses from published work.
Collection
Publications
Title
Recherche de l'amplification du gène HER2: la CISH est-elle une alternative à la technique FISH [HER2 gene amplification assay: is CISH an alternative to FISH?].
Journal
Annales De Pathologie
Author(s)
Denoux Y., Arnould L., Fiche M., Lannes B., Couturier J., Vincent-Salomon A., Penault-Llorca F., Antoine M., Balaton A., Baranzelli M.C., Becette V., Bellocq J.P., Bibeau F., Ettore F., Fridman V., Gnassia J.P., Jacquemier J., MacGrogan G., Mathieu M.C., Migeon C., Rigaud C., Roger P., Sigal-Zafrani B., Simony-Lafontaine J., Trassard M., Treilleux I., Verriele V., Voigt J.J.
Working group(s)
Groupe d'Etude des Facteurs Pronostiques en Immunohistochimie dans le Cancer du Sein
ISSN
0242-6498 (Print)
ISSN-L
0242-6498
Publication state
Published
Issued date
2003
Volume
23
Number
6
Pages
617-622
Language
french
Notes
Publication types: Comparative Study ; English Abstract ; Evaluation Studies ; Journal Article ; Review
Abstract
The HER2 proto-oncogene encodes a transmembrane protein, which is considered to function as a growth factor receptor. Overexpression of this protein found by immunohistochemistry in about 20% of infiltrating breast carcinomas, has a predictive value of response to treatment by trastuzumab, an anti-HER2 humanized monoclonal antibody. Search for HER2 gene amplification is necessary to adapt the immunohistochemical technique quality and also in the cases of delicate analysis or weak overexpression. It is usually carried out by Fluorescence In Situ Hybridization (FISH). A more recent hybridization technique, named CISH because of its chromogenic revelation is an alternative method, which gives highly correlated results with FISH. We present details of this technique, which may be more familiar for the pathologists than FISH, because reading analysis is similar to that of immunohistochemical staining.
Keywords
Breast Neoplasms/chemistry, Breast Neoplasms/genetics, Carcinoma, Ductal, Breast/chemistry, Carcinoma, Ductal, Breast/genetics, Chromogenic Compounds/analysis, Chromosomes, Human, Pair 17/genetics, DNA Probes, Digoxigenin/analysis, Female, Genes, erbB-2, Humans, In Situ Hybridization/methods, In Situ Hybridization, Fluorescence, Nucleic Acid Amplification Techniques, Specimen Handling
Pubmed
Create date
04/11/2010 10:34
Last modification date
20/08/2019 15:51
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