Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses

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State: Serval
Version: author
Serval ID
serval:BIB_D1AFA81DDA61
Type
Article: article from journal or magazin.
Collection
Publications
Title
Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses
Journal
Journal of Experimental Medicine
Author(s)
Precopio  M. L., Betts  M. R., Parrino  J., Price  D. A., Gostick  E., Ambrozak  D. R., Asher  T. E., Douek  D. C., Harari  A., Pantaleo  G., Bailer  R., Graham  B. S., Roederer  M., Koup  R. A.
ISSN
0022-1007 (Print)
Publication state
Published
Issued date
06/2007
Volume
204
Number
6
Pages
1405-16
Notes
Journal Article
Research Support, N.I.H., Intramural --- Old month value: Jun 11
Abstract
Vaccinia virus immunization provides lifelong protection against smallpox, but the mechanisms of this exquisite protection are unknown. We used polychromatic flow cytometry to characterize the functional and phenotypic profile of CD8(+) T cells induced by vaccinia virus immunization in a comparative vaccine trial of modified vaccinia virus Ankara (MVA) versus Dryvax immunization in which protection was assessed against subsequent Dryvax challenge. Vaccinia virus-specific CD8(+) T cells induced by both MVA and Dryvax were highly polyfunctional; they degranulated and produced interferon gamma, interleukin 2, macrophage inflammatory protein 1beta, and tumor necrosis factor alpha after antigenic stimulation. Responding CD8(+) T cells exhibited an unusual phenotype (CD45RO(-)CD27(intermediate)). The unique phenotype and high degree of polyfunctionality induced by vaccinia virus also extended to inserted HIV gene products of recombinant NYVAC. This quality of the CD8(+) T cell response may be at least partially responsible for the profound efficacy of these vaccines in protection against smallpox and serves as a benchmark against which other vaccines can be evaluated.
Pubmed
Web of science
Open Access
Yes
Create date
25/01/2008 16:00
Last modification date
09/05/2019 1:36
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