Effect of germination on the proteinase activities detected in Soybean cotyledons
Details
Request a copy Under indefinite embargo.UNIL restricted access
State: Public
Version: author
Serval ID
serval:BIB_D172645770B1
Type
Inproceedings: an article in a conference proceedings.
Publication sub-type
Abstract (Abstract): shot summary in a article that contain essentials elements presented during a scientific conference, lecture or from a poster.
Collection
Publications
Institution
Title
Effect of germination on the proteinase activities detected in Soybean cotyledons
Title of the conference
13th Annual Meeting of the Union of Swiss Societies of Experimental Biology
Address
Lausanne, Switzerland, March 26-27, 1981
ISBN
0014-4754
Publication state
Published
Issued date
1981
Peer-reviewed
Oui
Volume
37
Series
Experientia
Pages
626
Language
english
Abstract
Soybeans (Glycine max. var. Altona) were germinated for up to 4 days. Radicles , teguments and epicotyls were then removed and aqueous extracts were obtained from freezedried, ground and defatted cotyledons. The proteolytic activity of the soymeal extracts was investigated using 16
different synthetic and natural substrates. Neutral and acid proteinases were detected, as well as aminopeptidases and carboxypeptidases. Comparison between germinated (1, 2, 3 and 4 days) and ungerminated cotyledons showed a dramatic increase of neutra! proteinase and carboxypeptidase C activities with germination, whereas acid proteinase and aminopeptidase activities decreased significantly from the first day of germination. Preliminary results demon-strated that a minimum of 3 molecular species were responsible for the aminopeptidase activity, whereas molecular weight monodispersity characterized the neutral proteinases.
different synthetic and natural substrates. Neutral and acid proteinases were detected, as well as aminopeptidases and carboxypeptidases. Comparison between germinated (1, 2, 3 and 4 days) and ungerminated cotyledons showed a dramatic increase of neutra! proteinase and carboxypeptidase C activities with germination, whereas acid proteinase and aminopeptidase activities decreased significantly from the first day of germination. Preliminary results demon-strated that a minimum of 3 molecular species were responsible for the aminopeptidase activity, whereas molecular weight monodispersity characterized the neutral proteinases.
Web of science
Create date
13/08/2015 9:01
Last modification date
20/08/2019 16:51
Usage data
