Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence

Details

Serval ID
serval:BIB_D0ABC4A2B3BC
Type
Article: article from journal or magazin.
Collection
Publications
Title
Identification of biofilm-associated cluster (bac) in Pseudomonas aeruginosa involved in biofilm formation and virulence
Journal
PLoS One
Author(s)
Mace C., Seyer D., Chemani C., Cosette P., Di-Martino P., Guery B., Filloux A., Fontaine M., Molle V., Junter G. A., Jouenne T.
ISSN
1932-6203 (Electronic)
ISSN-L
1932-6203
Publication state
Published
Issued date
2008
Volume
3
Number
12
Pages
e3897
Language
english
Notes
Mace, Camille
Seyer, Damien
Chemani, Chanez
Cosette, Pascal
Di-Martino, Patrick
Guery, Benoit
Filloux, Alain
Fontaine, Marc
Molle, Virginie
Junter, Guy-Alain
Jouenne, Thierry
eng
Research Support, Non-U.S. Gov't
PLoS One. 2008;3(12):e3897. doi: 10.1371/journal.pone.0003897. Epub 2008 Dec 9.
Abstract
Biofilms are prevalent in diseases caused by Pseudomonas aeruginosa, an opportunistic and nosocomial pathogen. By a proteomic approach, we previously identified a hypothetical protein of P. aeruginosa (coded by the gene pA3731) that was accumulated by biofilm cells. We report here that a Delta pA3731 mutant is highly biofilm-defective as compared with the wild-type strain. Using a mouse model of lung infection, we show that the mutation also induces a defect in bacterial growth during the acute phase of infection and an attenuation of the virulence. The pA3731 gene is found to control positively the ability to swarm and to produce extracellular rhamnolipids, and belongs to a cluster of 4 genes (pA3729-pA3732) not previously described in P. aeruginosa. Though the protein PA3731 has a predicted secondary structure similar to that of the Phage Shock Protein, some obvious differences are observed compared to already described psp systems, e.g., this unknown cluster is monocistronic and no homology is found between the other proteins constituting this locus and psp proteins. As E. coli PspA, the amount of the protein PA3731 is enlarged by an osmotic shock, however, not affected by a heat shock. We consequently named this locus bac for biofilm-associated cluster.
Keywords
Amino Acid Sequence, Animals, Bacterial Adhesion, Bacterial Proteins/chemistry/genetics/*metabolism, Biofilms/*growth & development, Biological Assay, Cell Line, Tumor, Computational Biology, Genes, Bacterial, Glycolipids/metabolism, Heat-Shock Response, Humans, Kinetics, Male, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Multigene Family, Mutation/genetics, Osmotic Pressure, Pseudomonas Infections/genetics, Pseudomonas aeruginosa/genetics/growth & development/*pathogenicity/*physiology, Surface Properties, Virulence
Pubmed
Create date
29/04/2021 10:59
Last modification date
30/04/2021 6:38
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