Improved long-term outcome after transient cerebral ischemia in aquaporin-4 knockout mice.
Details
Serval ID
serval:BIB_D057B20A1E80
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Improved long-term outcome after transient cerebral ischemia in aquaporin-4 knockout mice.
Journal
Journal of cerebral blood flow and metabolism
ISSN
1559-7016 (Electronic)
ISSN-L
0271-678X
Publication state
Published
Issued date
01/2017
Peer-reviewed
Oui
Volume
37
Number
1
Pages
277-290
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
A hallmark of stroke is water accumulation (edema) resulting from dysregulation of osmotic homeostasis. Brain edema contributes to tissue demise and may lead to increased intracranial pressure and lethal herniation. Currently, there are only limited treatments to prevent edema formation following stroke. Aquaporin 4 (AQP4), a brain water channel, has become a focus of interest for therapeutic approaches targeting edema. At present, there are no pharmacological tools to block AQP4. The role of AQP4 in edema after brain injury remains unclear with conflicting results from studies using AQP4(-/-) mice and of AQP4 expression following stroke. Here, we studied AQP4 and its role in edema formation by testing AQP4(-/-) mice in a model of middle cerebral artery occlusion using novel quantitative MRI water content measurements, histology and behavioral changes as outcome measures. Absence of AQP4 was associated with decreased mortality and increased motor recovery 3 to 14 days after stroke. Behavioral improvement was associated with decreased lesion volume, neuronal cell death and neuroinflammation in AQP4(-/-) compared to wild type mice. Our data suggest that the lack of AQP4 confers an overall beneficial role at long term with improved neuronal survival and reduced neuroinflammation, but without a direct effect on edema formation.
Keywords
Animals, Aquaporin 4/deficiency, Aquaporin 4/genetics, Aquaporin 4/pharmacology, Brain Edema/diagnostic imaging, Brain Edema/etiology, Brain Edema/prevention & control, Brain Ischemia/complications, Brain Ischemia/therapy, Cell Death/drug effects, Encephalitis, Infarction, Middle Cerebral Artery, Ischemic Attack, Transient/complications, Ischemic Attack, Transient/therapy, Magnetic Resonance Imaging, Mice, Mice, Knockout, Neurons/pathology, Treatment Outcome, Aquaporin, astrocyte, blood-brain barrier, stroke, transient middle cerebral artery occlusion
Pubmed
Web of science
Create date
20/01/2016 16:28
Last modification date
20/08/2019 16:50