Absence of central circadian pacemaker abnormalities in humans with loss of function mutation in prokineticin 2.

Details

Serval ID
serval:BIB_D0377FD3B492
Type
Article: article from journal or magazin.
Collection
Publications
Title
Absence of central circadian pacemaker abnormalities in humans with loss of function mutation in prokineticin 2.
Journal
Journal of Clinical Endocrinology and Metabolism
Author(s)
Balasubramanian R., Cohen D.A., Klerman E.B., Pignatelli D., Hall J.E., Dwyer A.A., Czeisler C.A., Pitteloud N., Crowley W.F.
ISSN
1945-7197 (Electronic)
ISSN-L
0021-972X
Publication state
Published
Issued date
2014
Peer-reviewed
Oui
Volume
99
Number
3
Pages
E561-E566
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., ExtramuralPublication Status: ppublish
Abstract
CONTEXT: Loss of prokineticin 2 (PROK2) signaling in mice disrupts circadian rhythms, but the role of PROK2 signaling in the regulation of circadian rhythms in humans is undetermined.
OBJECTIVE: The aim of the study was to examine the circadian rhythms of humans with a complete loss-of-function PROK2 mutation using an inpatient constant routine (CR) protocol.
DESIGN AND SETTING: We conducted a case study in an academic medical center.
SUBJECTS AND METHODS: Two siblings (one male and one female, ages 67 and 62 y, respectively) with isolated GnRH deficiency (IGD) due to a biallelic loss-of-function PROK2 mutation were studied using an inpatient CR protocol. Historical data from inpatient CR protocols conducted in healthy controls (ages 65-81 y) were used for comparison.
MAIN OUTCOME MEASURES: We measured circadian phase markers (melatonin, cortisol, and core body temperature) and neurobehavioral performance (psychomotor vigilance task [PVT] and subjective alertness scale).
RESULTS: Circadian waveforms of melatonin and cortisol did not differ between the IGD participants with PROK2 mutation and controls. In both IGD participants, neurobehavioral testing with PVT showed disproportionate worsening of PVT lapses and median reaction time in the second half of the CR.
CONCLUSIONS: Humans with loss of PROK2 signaling lack abnormalities in circadian phase markers, indicating intact central circadian pacemaker activity in these patients. These results suggest that PROK2 signaling in humans is not required for central circadian pacemaker function. However, impaired PVT in the PROK2-null participants despite preserved endocrine rhythms suggests that PROK2 may transmit circadian timing information to some neurobehavioral neural networks.
Keywords
Aged, Aged, 80 and over, Biological Clocks/genetics, Case-Control Studies, Chronobiology Disorders/complications, Chronobiology Disorders/epidemiology, Codon, Nonsense, Female, Gastrointestinal Hormones/genetics, Gonadotropin-Releasing Hormone/deficiency, Gonadotropin-Releasing Hormone/genetics, Humans, Hypogonadism/complications, Hypogonadism/epidemiology, Male, Middle Aged, Neuropeptides/genetics, Siblings, Sleep/genetics
Pubmed
Open Access
Yes
Create date
13/08/2014 10:22
Last modification date
20/08/2019 16:50
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