Cytolytic T lymphocyte precursor cells in congenitally athymic C57BL/6 nu/nu mice: quantitation, enrichment, and specificity

Details

Serval ID
serval:BIB_D0091872D7F2
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cytolytic T lymphocyte precursor cells in congenitally athymic C57BL/6 nu/nu mice: quantitation, enrichment, and specificity
Journal
Journal of Immunology
Author(s)
Maryanski  J. L., MacDonald  H. R., Sordat  B., Cerottini  J. C.
ISSN
0022-1767 (Print)
Publication state
Published
Issued date
03/1981
Volume
126
Number
3
Pages
871-6
Notes
Journal Article --- Old month value: Mar
Abstract
A sensitive limiting dilution microculture system was used to obtain minimal estimates of the frequency of CTL precursor cells (CTL-P) in spleens from 5- to 14-mo-old C57BL/6 nu/nu mice. Frequency determinations of CTL-P directed against H-2d alloantigens ranged from 1/159,000 to 1/12,400. The relatively low frequency of CTL-P was enriched nearly 10-fold (to 1/2300) by passage of nude spleen cells over a column of nylon wool. After priming nude spleen cells for 7 days in conventional MLC, 1 to 3% of the MLC cells could be operationally identified as CTL-P. Furthermore, the progeny of MLC-primed nude CTL-P were specifically cytolytic for target cells of the strain used for priming. Such a system may be useful for analyzing the specificity repertoires of cells of the T cell lineage that have not undergone thymic influence.
Keywords
Aging Animals Cell Differentiation Cell Separation *Cytotoxicity, Immunologic Lymphocyte Culture Test, Mixed Mice Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Mice, Inbred DBA Mice, Nude/*immunology Spleen/immunology T-Lymphocytes/*cytology/immunology
Pubmed
Web of science
Create date
28/01/2008 12:14
Last modification date
20/08/2019 16:50
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