Promyelocytic leukemia protein in retinoic acid-induced chromatin remodeling of Oct4 gene promoter.
Details
Serval ID
serval:BIB_CFEFC5ED8B13
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Promyelocytic leukemia protein in retinoic acid-induced chromatin remodeling of Oct4 gene promoter.
Journal
Stem cells
ISSN
1549-4918 (Electronic)
ISSN-L
1066-5099
Publication state
Published
Issued date
04/2011
Peer-reviewed
Oui
Volume
29
Number
4
Pages
660-669
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Promyelocytic leukemia (Pml) protein is required for Oct4 gene expression and the maintenance of its open chromatin conformation in stem cells. In proliferating stem cells, Pml-nuclear body, along with transcription factors TR2, steroidogenic factor 1 (SF1) and Sp1, and Brg1-dependent chromatin remodeling complex (BRGC), associates with conserved region 1 (CR1) of this promoter to maintain a nucleosome-free region for gene activity. Retinoic acid (RA) rapidly downregulates Pml, resulting in the replacement of BRGC with Brm-containing remodeling complex, disassociation of SF1 and Sp1, retaining of TR2, recruitment of receptor-interaction protein 140, G9a and HP1γ, and sequential insertion of two nucleosomes on CR1 that progressively displays repressive heterochromatin marks. This study demonstrates a functional role for Pml in maintaining a specific open chromatin conformation of the Oct4 promoter region for its constant expression in stem cells; and illustrates the mechanism underlying RA-induced chromatin remodeling of Oct4 gene in differentiating cells, in which Pml plays a critical role. The study also demonstrates a novel mode of chromatin remodeling, which occurs by repositioning and sequentially inserting nucleosomes into a specific region of the gene promoter to compact the chromatin in differentiating cells.
Keywords
Animals, Cell Differentiation/genetics, Chromatin Assembly and Disassembly/genetics, Chromatin Immunoprecipitation, Gene Expression Regulation, Developmental, Intracellular Signaling Peptides and Proteins/metabolism, Mice, Nuclear Proteins/biosynthesis, Nuclear Proteins/metabolism, Nucleosomes, Octamer Transcription Factor-3/genetics, Promoter Regions, Genetic, Promyelocytic Leukemia Protein, RNA Interference, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, Sp1 Transcription Factor/metabolism, Steroidogenic Factor 1/metabolism, Transcription Factors/biosynthesis, Transcription Factors/metabolism, Tretinoin/metabolism, Tumor Suppressor Proteins/biosynthesis, Tumor Suppressor Proteins/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
05/04/2019 16:34
Last modification date
20/08/2019 16:50