Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients.
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State: Public
Version: Final published version
State: Public
Version: Final published version
Serval ID
serval:BIB_CFDAD27012D4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Detection of functional antigen-specific T cells from urine of non-muscle invasive bladder cancer patients.
Journal
Oncoimmunology
ISSN
2162-4011 (Print)
Publication state
Published
Issued date
2012
Volume
1
Number
5
Pages
694-698
Language
english
Notes
Publication types: JOURNAL ARTICLEPublication Status: ppublish
Abstract
To gain further insights into the role of T lymphocytes in immune responses against bladder tumors, we developed a method that monitors the presence of functional antigen-specific T cells in the urine of non-muscle invasive bladder cancer patients. As relatively few immune cells can usually be recovered from urine, we examined different isolation/amplification protocols and took advantage of patients treated with weekly intravesical instillations of Bacillus Calmette-Guérin, resulting in large amounts of immune cells into urine. Our findings demonstrate that, upon in vitro amplification, antigen-specific T cells can be detected by an interferon γ (IFNγ)-specific ELISPOT assay.
Pubmed
Create date
17/01/2013 17:19
Last modification date
20/08/2019 16:50