Polyadenylation of Na(+)-K(+)-ATPase beta 1-subunit during early development of Xenopus laevis

Details

Serval ID
serval:BIB_CFD011848AEF
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Polyadenylation of Na(+)-K(+)-ATPase beta 1-subunit during early development of Xenopus laevis
Journal
American Journal of Physiology
Author(s)
Burgener-Kairuz  P., Corthesy-Theulaz  I., Merillat  A. M., Good  P., Geering  K., Rossier  B. C.
ISSN
0363-6143
Publication state
Published
Issued date
01/1994
Volume
266
Number
1 Pt 1
Pages
C157-64
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan
Abstract
In fully grown Xenopus oocytes, the synthesis of beta-subunits is limiting for the formation of functional Na(+)-K(+)-adenosinetriphosphatase alpha/beta-complexes (Geering, K. FEBS Lett. 285: 189-193, 1991). In the present study, we show that during oocyte growth (from stage I to stage VI) alpha 1-, but not beta 1- or beta 3-isoform, mRNAs accumulate. In addition, beta-mRNAs are apparently sequestered in an untranslated pool in fully grown oocytes (stage VI). From fertilization to morulation, the total pools of alpha 1-, beta 1-, or beta 3-mRNAs vary little. Whereas polyadenylated [poly(A)+] alpha 1- and beta 3-isoform mRNAs did not change significantly, poly(A)+ beta 1-mRNA abundance increased three- to fourfold at morulation, accompanied by a parallel increase in beta 1-protein synthesis. After midblastula transition (i.e., at early gastrula) and during neurulation, poly(A)+ alpha 1- and beta 3-mRNAs accumulated rapidly, whereas poly(A)+ beta 1-mRNA accumulation was delayed by approximately 2 h, beginning only at early neurula. Our results indicate that 1) the abundance of poly(A)+ beta 1-mRNA is rate limiting during embryonic development for the assembly of alpha 1/beta 1-heterodimers, shown to be involved in the vectorial transport of sodium in kidney cells, and 2) the polyadenylation of beta 1-mRNA is a rate-limiting factor during morulation for the synthesis and assembly of new sodium pumps at the time of blastocoel fluid formation. The 3'-untranslated region of beta 1-mRNA (but not of alpha 1-mRNA) expresses cytoplasmic polyadenylation elements (CPEs) with the consensus sequence AXX-AUUUU(A/U)(A/U)(A/U). A role of CPE in the differential polyadenylation of alpha 1- and beta 1-mRNA is proposed.
Keywords
Aging/metabolism Amino Acid Sequence Animals Brain/enzymology Embryo, Nonmammalian/*metabolism/physiology *Embryonic and Fetal Development Female Male Molecular Sequence Data Na(+)-K(+)-Exchanging ATPase/chemistry/*metabolism Nucleic Acid Hybridization Oocytes/metabolism/physiology Poly A/*metabolism Protein Biosynthesis RNA, Messenger/metabolism Ribonuclease H, Calf Thymus Xenopus laevis
Pubmed
Web of science
Create date
24/01/2008 12:28
Last modification date
20/08/2019 15:50
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