Cortical and subcortical distribution of ionotropic purinergic receptor subunit type 1 (P2X(1)R) immunoreactive neurons in the rat forebrain.

Details

Serval ID
serval:BIB_CF17D4EC3027
Type
Article: article from journal or magazin.
Collection
Publications
Title
Cortical and subcortical distribution of ionotropic purinergic receptor subunit type 1 (P2X(1)R) immunoreactive neurons in the rat forebrain.
Journal
Neuroscience
Author(s)
Florenzano F., Carrive P., Viscomi M.T., Ferrari F., Latini L., Conversi D., Cabib S., Bagni C., Molinari M.
ISSN
0306-4522 (Print)
ISSN-L
0306-4522
Publication state
Published
Issued date
06/02/2008
Volume
151
Number
3
Pages
791-801
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Ionotropic purinergic receptors (P2XR) are ATP-gated cationic channels composed of seven known subunits (P2X(1-7)R) and involved in different functions in neural tissue. Although their presence has been demonstrated in the brain, few studies have investigated their expression pattern. In particular, ionotropic purinergic receptor subunit type 1 (P2X(1)R) has been observed in the cerebellum and in brainstem nuclei. The present study investigates the P2X(1)R expression pattern in the rat forebrain using immunohistochemistry. The specificity of the immunolabeling has been verified by Western blotting and in situ hybridization methods. P2X(1)R immunoreactivity was specifically localized in neurons, dendrites and axons throughout the forebrain. Characteristic differences in the distribution of P2X(1)R were observed in different cortical areas. In prefrontal, cingulate and perirhinal cortices, very intense labeling was present in neuronal bodies. In frontal, parietal, temporal and occipital cortices, immunostaining was lighter and mainly found in dendrites and axons. The hippocampal formation was intensely labeled. Labeling was present almost exclusively in dendrites and axons and never in neuronal bodies. The diencephalon was devoid of P2X(1)R positive neurons or fibers except for the medial habenular nucleus, which showed very intense P2X(1)R immunostaining. Furthermore, two subcortical regions, namely, the nucleus centralis of the amygdala and the bed nucleus of the stria terminalis, showed intense P2X(1)R neuronal labeling. Present data indicate that P2X(1)R are prevalent in forebrain areas involved in the integration of cognitive, limbic and autonomic functions.

Keywords
Animals, Glial Fibrillary Acidic Protein/metabolism, Male, Neurons/metabolism, Neurons/ultrastructure, Phosphopyruvate Hydratase/metabolism, Prosencephalon/cytology, RNA, Messenger/metabolism, Rats, Rats, Wistar, Receptors, Purinergic P2/genetics, Receptors, Purinergic P2/metabolism, Receptors, Purinergic P2X
Pubmed
Create date
06/03/2017 18:23
Last modification date
20/08/2019 16:49
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