Leishmania major infection in mice primes for specific major histocompatibility complex class I-restricted CD8+ cytotoxic T cell responses

Details

Serval ID
serval:BIB_CF0E7946E0D3
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Leishmania major infection in mice primes for specific major histocompatibility complex class I-restricted CD8+ cytotoxic T cell responses
Journal
European Journal of Immunology
Author(s)
da Conceicao-Silva  F., Perlaza  B. L., Louis  J. A., Romero  P.
ISSN
0014-2980 (Print)
Publication state
Published
Issued date
11/1994
Volume
24
Number
11
Pages
2813-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Nov
Abstract
This report shows that lymphoid tissues of mice which have resolved a primary infection with Leisihmania major contain parasite-specific major histocompatibility complex (MHC) class I-restricted cytolytic CD8+ T cell precusors that can be expanded after specific restimulation in vitro with syngeneic antigen-presenting cells pulsed with a cyanogen bromide digest of L. major. In H-2b mice, two distinct populations of CD8+ T cells were identified which both lysed target cells pulsed with L. major-derived peptides but were restricted by a different H-2b class I gene product. Interestingly, these two populations appear to recognize different parasite-derived peptides. It is noteworthy that one Kb-restricted CD8+ T cell line was able to specifically lyse syngeneic macrophages infected with viable L. major, indicating that some L. major-derived peptides may reach the MHC class I pathway of presentation from the phagolysosomal compartment where the parasites are confined in infected macrophages. The importance of these parasite-specific MHC class I restricted cytolytic CD8+ T cells for the elimination of L. major by the infected host remains to be determined.
Keywords
Animals Cell Line Female Histocompatibility Antigens Class I/*immunology Interferon Type II/biosynthesis *Leishmania major Leishmaniasis, Cutaneous/*immunology Macrophages/immunology Mice Mice, Inbred C57BL T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Create date
28/01/2008 11:28
Last modification date
20/08/2019 15:49
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