Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy.
Details
Serval ID
serval:BIB_CEFF3604A4EB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy.
Journal
Optics express
ISSN
1094-4087 (Electronic)
ISSN-L
1094-4087
Publication state
Published
Issued date
10/09/2012
Peer-reviewed
Oui
Volume
20
Number
19
Pages
21385-21399
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed inherent to OCM. We report on the detection of single 40 nm gold particles with a 0.5 μm lateral and 2 μm axial resolution over a 50 μm depth of field and the three-dimensional localization of gold colloids within living cells. In combination with intrinsic sample contrast measured with dark-field OCM, poli-OCM offers a versatile platform for functional cell imaging.
Keywords
Cell Survival, Dimethylpolysiloxanes/chemistry, Gold/chemistry, HeLa Cells, Humans, Imaging, Three-Dimensional/methods, Metal Nanoparticles/chemistry, Microscopy/methods, Optical Phenomena, Signal-To-Noise Ratio, Temperature
Pubmed
Web of science
Open Access
Yes
Create date
22/10/2014 8:02
Last modification date
01/03/2024 16:13