Single-cell CUT&Tag analysis of chromatin modifications in differentiation and tumor progression.
Details
Serval ID
serval:BIB_CE7A12D8A905
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Single-cell CUT&Tag analysis of chromatin modifications in differentiation and tumor progression.
Journal
Nature biotechnology
ISSN
1546-1696 (Electronic)
ISSN-L
1087-0156
Publication state
Published
Issued date
07/2021
Peer-reviewed
Oui
Volume
39
Number
7
Pages
819-824
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Abstract
Methods for quantifying gene expression <sup>1</sup> and chromatin accessibility <sup>2</sup> in single cells are well established, but single-cell analysis of chromatin regions with specific histone modifications has been technically challenging. In this study, we adapted the CUT&Tag method <sup>3</sup> to scalable nanowell and droplet-based single-cell platforms to profile chromatin landscapes in single cells (scCUT&Tag) from complex tissues and during the differentiation of human embryonic stem cells. We focused on profiling polycomb group (PcG) silenced regions marked by histone H3 Lys27 trimethylation (H3K27me3) in single cells as an orthogonal approach to chromatin accessibility for identifying cell states. We show that scCUT&Tag profiling of H3K27me3 distinguishes cell types in human blood and allows the generation of cell-type-specific PcG landscapes from heterogeneous tissues. Furthermore, we used scCUT&Tag to profile H3K27me3 in a patient with a brain tumor before and after treatment, identifying cell types in the tumor microenvironment and heterogeneity in PcG activity in the primary sample and after treatment.
Keywords
Brain Neoplasms/genetics, Brain Neoplasms/metabolism, Cell Differentiation, Chromatin/genetics, Chromatin/physiology, Embryonic Stem Cells, Gene Expression Regulation, Gene Silencing, Humans, Jumonji Domain-Containing Histone Demethylases/genetics, Jumonji Domain-Containing Histone Demethylases/metabolism, K562 Cells, Polycomb-Group Proteins/genetics, Polycomb-Group Proteins/metabolism, Single-Cell Analysis
Pubmed
Web of science
Create date
28/02/2022 12:45
Last modification date
23/03/2024 8:24