Epidermal growth factor is a neuronal differentiation factor for retinal stem cells in vitro
Details
Serval ID
serval:BIB_CE637A4CBEE4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Epidermal growth factor is a neuronal differentiation factor for retinal stem cells in vitro
Journal
Stem Cells
ISSN
1066-5099 (Print)
Publication state
Published
Issued date
03/2006
Volume
24
Number
3
Pages
696-706
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
Stem cells are a tool for in vitro elucidation of the putative role of factors on cell fate. Herein we analyze the role of epidermal growth factor (EGF) on progeny derived from retinal stem cells (RSCs). We isolated cells from neuroretinas of neonate mice. All the proliferating cells harbored the radial glia marker RC2, expressed transcription factors usually found in radial glia (Mash1, Pax6), and met the criteria of stem cells: high capacity of expansion, maintenance of an undifferentiated state, and multipotency demonstrated by clonal analysis. We analyzed the differentiation 7 days after transfer of the cells in different culture media. In absence of serum, EGF led to the expression of the neuronal marker beta-tubulin-III and acquisition of neuronal morphology in 15% of the cells. Analysis of cell proliferation by bromodeoxyuridine incorporation revealed that EGF mainly induced the formation of neurons without stimulating cell cycle progression. Moreover, a pulse of 2-hour EGF stimulation was sufficient to induce neuronal differentiation. Some neurons were committed to the retinal ganglion cell (RGC) phenotype, as revealed by the expression of retinal ganglion markers (Ath5, Brn3b, and melanopsin) and in a few cases to other retinal phenotypes (photoreceptors [PRs] and bipolar cells). We confirmed that the late RSCs were not restricted over time and that they conserved their multipotency by generating retinal phenotypes that usually appear at early (RGC) or late (PRs) developmental stages. Our results show that EGF is not only a factor controlling glial development, as previously shown, but also a potent differentiation factor for retinal neurons, at least in vitro.
Keywords
Animals
Animals, Newborn
Antigens, Differentiation/biosynthesis
Cell Differentiation/*drug effects/physiology
Cells, Cultured
Epidermal Growth Factor/metabolism/*pharmacology
Gene Expression Regulation/*drug effects/physiology
Humans
Mice
Neuroglia/cytology/physiology
Retinal Ganglion Cells/cytology/*physiology
Stem Cells/cytology/*physiology
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 13:58
Last modification date
20/08/2019 16:48